User:Kelli B. Pointer

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(Module 3 Project Proposal (work in progress))
 
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{{New user}}
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==Contact Info==
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[[Image:OWWEmblem.png|thumb|right|Kelli B. Pointer (an artistic interpretation)]]
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[[Image:Me_senior_pic.jpg]]
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*Kelli B. Pointer
 
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*MIT
 
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*Flossmoor,IL
 
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*kpointer AT mit DOT edu
 
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I am a member of the spring 2008 20.109 lab course
 
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==Education==
 
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<!--Include info about your educational background-->
 
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* 2006, Homewood-Flossmoor High School
 
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* 2010, BS, Massachusetts Institute of Technology
 
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==Research interests==
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<font size="4">Kelli Pointer</font size>
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<!-- Feel free to add brief descriptions to your research interests as well -->
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# pharmaceuticals
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# oncology
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# stem cells
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==Publications==
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<font size="2">Department of Biological Engineering<br>
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Coming soon!!
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New House 2<br>
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472 Memorial Dr.<br>
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Cambridge, MA 02139 <br>
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==Useful links==
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kpointer AT mit DOT edu
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*[[OpenWetWare:Welcome|Introductory tutorial]]
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*[[Help|OpenWetWare help pages]]
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Please copy the source code from this page to your user page, fill in the answers and print out a copy for next time.<br>  
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</font size>
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You do not need to keep the information on your user page once you've printed it out.
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==Registration/Questionnaire: 20.109 Spring 2008==
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===Last Name===
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This website is for my 20.109 class, Laboratory Fundamentals of Biological Engineering.
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Pointer
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===First Name===
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== Module 3 Project Proposal ==
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Kelli
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===Project Title===
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===Preferred name===
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Storage of stem cells for later differentiation into cardiovascular vessels
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Kelli
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=== Background Information ===
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===Course/Minor===
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Many problems arise when things like organs and drugs are introduced into the body because the body either rejects whatever is being introduced or the thing that is being introduced rejects the body.  For this reason, it is ideal to use something that comes directly from someone's body to avoid immune responses and ultimately rejection.  Thus, if a method is created where stem cells can be isolated in the fetal stage or even from the umbilical code, then methods to grow organs or vessels from those stem cells can be implemented and rejection would decrease dramatically.
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Major: Biological Engineering
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=== Project Overview ===
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Minor: Brain and Cognitive Sciences
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===Year of Graduation===
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2010
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===Telephone #===
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857-272-5220
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===Email===
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kpointer AT mit DOT edu
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===Have you taken===
 
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7.05/5.07 (Biochemistry)<br>
 
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7.06 (Cell Biology)<br>
 
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7.02 (General Biology Lab)<br>
 
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5.310 (General Chemistry Lab)<br>
 
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Have you taken any of these classes?
 
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Not yet
 
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Do you have any experience culturing cells (mammalian, yeast or microbial)?<br>  Yes  No
 
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I have experience culturing yeast and microbial cells
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=== Project Problems ===
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===Project Goals===
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Create a method that can store umbilical cord stem cells or fetal stem cells for later use if problems arise in the cardiovascular system.  Then, ultimately use those stem cells for in vitro growth of vessels.
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=== Details and Methods ===
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=== Predicted Outcomes ===
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===References ===
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Do you have any experience in molecular biology (electrophoresis, PCR, etc)?<br>  Yes  No
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Blattmann, Annette et al., The formation of pores in the basal lamina of regenerated renal tubules                Biomaterials (2008)
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I have experience with gel electrophoresis and PCR
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In this assay they looked at the basal aspects of renal tubules generated at the Interphase of an artificial interstitium in order to gain more knowledge about the generation of renal tubules.  They took progenitor cells from neonatal rabbit kidney and put them inside a specific tissue holder that was covered by polyester fleece.  They found tissue-specific antibodies that showed that the tubes were completely covered by a basal lamina.  The matrix that was formed had three categories of pores which were widely distributed. The pores were also found in collections of duct tubules of the neonatal rabbit kidney.
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===Please briefly describe any previous laboratory experience===
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I participated in summer research at Brown University that focused on recombinant DNA.
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Currently I have a UROP in the Langer LabMy project is focusing on how hydrogels can be used as scaffolding for stem cells as well as a delivery mechanism into the body
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===Anything else you would like us to know?===
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Heydarkhan-Hagvall,Sepideh et al., Three-dimensional electrospun ECM-based hybrid scaffolds for cardiovascular tissue engineering, Biomaterials(2008)
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I am very excited for this course.  I love lab and I am looking forward to learning a lot from this class.
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Electrospinning can be used to take natural proteins or synthetic polymers to create fibrous scaffolds for tissue engineering.  In order to try to overcome the problems of scaffolding that is electospun from natural proteins, in this assay they determined characteristics of a scaffold composed of collagen, elastin, and gelatin in order to avoid chemical  cross-linking.  They found that fiber size increased and pore size decreased when the polymer concentrations were increasedThe tensile strength was less when compared to the traditional scaffolding that is made from natural proteins.  Ultimately, they found that combining synthetic polymers and natural proteins to create a scaffold was biologically and mechanically favorable
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G. Liu et al., Tissue-engineered bone formation with cryopreserved human bone marrow mesenchymal stem cells, Cryobiology (2008), doi:10.1016/ j.cryobiol.2008.02.008
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In this assay they tested to see if cryopreserved human bone marrow stem cells could maintain their potential for proliferation and osteogenic differentiation in vitro.  They slowly cooled bone marrow mesenchymal stem cells with Me2SO as a cyroprotectant and rapidly thawed it.  Then they froze the cells with liquid nitrogen for twenty four hours and thawed them. After they thawed the stem cells, they plated them and let them grow for four weeks.  After four weeks, they saw no difference in the growth rate or morphology of the cells that had been cryopreserved compared to the same cells that had not been cryopreserved.  They also found that the cryopreserved cells attached to scaffolding the same way that cells that were not cryopreserved did.  There were no significant differences between the cells that were cryopreserved and the cells that were not.
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== Research Interests ==
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<blockquote>
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*Cancer
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*Stem Cells
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*Tissue Engineering
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</blockquote>
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== Education==
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Massachusetts Institute of Technology, 2010<br>
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Homewood-Flossmoor High School, 2006<br>
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== Publications ==
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Coming soon!!!
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==Useful links==
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*[[OpenWetWare:Welcome|Introductory tutorial]]
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*[[Help|OpenWetWare help pages]]

Current revision

I am a new member of OpenWetWare!

Image:Me_senior_pic.jpg



Kelli Pointer

Department of Biological Engineering
New House 2
472 Memorial Dr.
Cambridge, MA 02139

kpointer AT mit DOT edu

This website is for my 20.109 class, Laboratory Fundamentals of Biological Engineering.

Contents

Module 3 Project Proposal

Project Title

Storage of stem cells for later differentiation into cardiovascular vessels

Background Information

Many problems arise when things like organs and drugs are introduced into the body because the body either rejects whatever is being introduced or the thing that is being introduced rejects the body. For this reason, it is ideal to use something that comes directly from someone's body to avoid immune responses and ultimately rejection. Thus, if a method is created where stem cells can be isolated in the fetal stage or even from the umbilical code, then methods to grow organs or vessels from those stem cells can be implemented and rejection would decrease dramatically.

Project Overview

Project Problems

Project Goals

Create a method that can store umbilical cord stem cells or fetal stem cells for later use if problems arise in the cardiovascular system. Then, ultimately use those stem cells for in vitro growth of vessels.

Details and Methods

Predicted Outcomes

References

Blattmann, Annette et al., The formation of pores in the basal lamina of regenerated renal tubules Biomaterials (2008)

In this assay they looked at the basal aspects of renal tubules generated at the Interphase of an artificial interstitium in order to gain more knowledge about the generation of renal tubules. They took progenitor cells from neonatal rabbit kidney and put them inside a specific tissue holder that was covered by polyester fleece. They found tissue-specific antibodies that showed that the tubes were completely covered by a basal lamina. The matrix that was formed had three categories of pores which were widely distributed. The pores were also found in collections of duct tubules of the neonatal rabbit kidney.


Heydarkhan-Hagvall,Sepideh et al., Three-dimensional electrospun ECM-based hybrid scaffolds for cardiovascular tissue engineering, Biomaterials(2008)

Electrospinning can be used to take natural proteins or synthetic polymers to create fibrous scaffolds for tissue engineering. In order to try to overcome the problems of scaffolding that is electospun from natural proteins, in this assay they determined characteristics of a scaffold composed of collagen, elastin, and gelatin in order to avoid chemical cross-linking. They found that fiber size increased and pore size decreased when the polymer concentrations were increased. The tensile strength was less when compared to the traditional scaffolding that is made from natural proteins. Ultimately, they found that combining synthetic polymers and natural proteins to create a scaffold was biologically and mechanically favorable


G. Liu et al., Tissue-engineered bone formation with cryopreserved human bone marrow mesenchymal stem cells, Cryobiology (2008), doi:10.1016/ j.cryobiol.2008.02.008

In this assay they tested to see if cryopreserved human bone marrow stem cells could maintain their potential for proliferation and osteogenic differentiation in vitro. They slowly cooled bone marrow mesenchymal stem cells with Me2SO as a cyroprotectant and rapidly thawed it. Then they froze the cells with liquid nitrogen for twenty four hours and thawed them. After they thawed the stem cells, they plated them and let them grow for four weeks. After four weeks, they saw no difference in the growth rate or morphology of the cells that had been cryopreserved compared to the same cells that had not been cryopreserved. They also found that the cryopreserved cells attached to scaffolding the same way that cells that were not cryopreserved did. There were no significant differences between the cells that were cryopreserved and the cells that were not.

Research Interests

  • Cancer
  • Stem Cells
  • Tissue Engineering

Education

Massachusetts Institute of Technology, 2010

Homewood-Flossmoor High School, 2006

Publications

Coming soon!!!


Useful links

Personal tools