User:Keoni K. Gandall/Notebook/Halobacterium NCR-1 vector

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==Project Description/Abstract==
==Project Description/Abstract==
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* Place short description of project or notes regarding this project
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*Goal- Create a plasmid for Halobacterium NCR-1 that the common DIY scientist can 1 obtain and 2 use
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* I am trying to create a shuttle vector for Halobacterium NCR-1. To do this, I am synthesizing the HF2 origin of replication, then, using the gibson assembly, will put it into a custom vector as well, with pUC19 origin, chloramphenicol resistance gene, and the green fluorescent protein. The first tests will be "does chloramphenicol affect Halobacterium?". If it does not, then I will just have to find another resistance gene. This will also answer a number of questions such as does the salt concentration in the media affect the GFP protein.  
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* Questions - Can chloramphenicol "kill" Halobacterium?  Is the GFP protein affected by salt concentrations? How do we most efficiently transform Halobacterium NCR-1? More to be answered...
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*Change Log- Delete Does T7 promoter "work" in Halobacterium. No source of T7 polymerase gene.
==Notes==
==Notes==

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Project Description/Abstract

  • Goal- Create a plasmid for Halobacterium NCR-1 that the common DIY scientist can 1 obtain and 2 use


  • I am trying to create a shuttle vector for Halobacterium NCR-1. To do this, I am synthesizing the HF2 origin of replication, then, using the gibson assembly, will put it into a custom vector as well, with pUC19 origin, chloramphenicol resistance gene, and the green fluorescent protein. The first tests will be "does chloramphenicol affect Halobacterium?". If it does not, then I will just have to find another resistance gene. This will also answer a number of questions such as does the salt concentration in the media affect the GFP protein.
  • Questions - Can chloramphenicol "kill" Halobacterium? Is the GFP protein affected by salt concentrations? How do we most efficiently transform Halobacterium NCR-1? More to be answered...





  • Change Log- Delete Does T7 promoter "work" in Halobacterium. No source of T7 polymerase gene.

Notes

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  • Example: This project is currently on hold until further notice.


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