User:Keoni K. Gandall/Notebook/Halobacterium NCR-1 vector

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Current revision (23:21, 13 January 2013) (view source)
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==Project Description/Abstract==
==Project Description/Abstract==
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* I am trying to create a shuttle vector for Halobacterium NCR-1. To do this, I am synthesizing the HF2 origin of replication, then, using the gibson assembly, will put it into a custom vector as well, with pUC19 origin, chloramphenicol resistance gene, and the green fluorescent protein. This will answer a number of questions such as does the salt concentration in the media affect the GFP protein.
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*Goal- Create a plasmid for Halobacterium NCR-1 that the common DIY scientist can 1 obtain and 2 use
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* Lorem ipsum dolor sit amet, consectetuer adipiscing elit. Donec porta commodo tellus. Nam a est eget libero mollis tincidunt. Aliquam purus. Quisque nulla ligula, facilisis in, pulvinar sed, molestie a, quam. Vestibulum at pede. In in sem eget odio eleifend placerat. Phasellus ultricies felis quis sapien. Etiam molestie volutpat quam. Praesent pulvinar scelerisque mi. Nam mi urna, fringilla eu, mattis sed, venenatis id, nunc. Maecenas velit eros, congue ut, placerat in, ornare vel, sem. Aenean porta enim sit amet felis gravida posuere. Phasellus faucibus nibh et orci.
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* I am trying to create a shuttle vector for Halobacterium NCR-1. To do this, I am synthesizing the HF2 origin of replication, then, using the gibson assembly, will put it into a custom vector as well, with pUC19 origin, chloramphenicol resistance gene, and the green fluorescent protein. The first tests will be "does chloramphenicol affect Halobacterium?". If it does not, then I will just have to find another resistance gene. This will also answer a number of questions such as does the salt concentration in the media affect the GFP protein.  
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* Questions - Can chloramphenicol "kill" Halobacterium?  Is the GFP protein affected by salt concentrations? How do we most efficiently transform Halobacterium NCR-1? More to be answered...
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*Change Log- Delete Does T7 promoter "work" in Halobacterium. No source of T7 polymerase gene.
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Project Description/Abstract

  • Goal- Create a plasmid for Halobacterium NCR-1 that the common DIY scientist can 1 obtain and 2 use


  • I am trying to create a shuttle vector for Halobacterium NCR-1. To do this, I am synthesizing the HF2 origin of replication, then, using the gibson assembly, will put it into a custom vector as well, with pUC19 origin, chloramphenicol resistance gene, and the green fluorescent protein. The first tests will be "does chloramphenicol affect Halobacterium?". If it does not, then I will just have to find another resistance gene. This will also answer a number of questions such as does the salt concentration in the media affect the GFP protein.
  • Questions - Can chloramphenicol "kill" Halobacterium? Is the GFP protein affected by salt concentrations? How do we most efficiently transform Halobacterium NCR-1? More to be answered...





  • Change Log- Delete Does T7 promoter "work" in Halobacterium. No source of T7 polymerase gene.

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