User:Keoni K. Gandall/Notebook/Halobacterium NCR-1 vector

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Project Description/Abstract

  • I am trying to create a shuttle vector for Halobacterium NCR-1. To do this, I am synthesizing the HF2 origin of replication, then, using the gibson assembly, will put it into a custom vector as well, with pUC19 origin, chloramphenicol resistance gene, and the green fluorescent protein. This will answer a number of questions such as does the salt concentration in the media affect the GFP protein.
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