Not much has been going on lately. I ordered DMSO, and some stuff to filter sterilize though.
One of the reasons is I have been debating getting competent cells from NEB. Today I I realized that I had taken too much time thinking out it, and all the work to get there cells just for one transformation. So, instead, I am making competent cells using the CaCl method and the modified TSS method, and I will compare the data I get.