User:Kersten S. Rabe
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Revision as of 09:11, 10 May 2010
- Dipl. Biochem. Kersten S. Rabe
- TU Dortmund
- Otto-Hahn Strasse 6
- 44227 Dortmund, Germany
- Email me through OpenWetWare
I work as a Post-Doc in the Niemeyer lab at Dortmund University. In Dec. 2009 I finished my time as Doktorand with "summa cum laude" ("Ausgezeichnet")
- From June 2010 on I will join the group of Frances Arnold as a DAAD stipendiary
- 2009-present Post-Doc with Prof. Niemeyer
- 2005-2009 phD work in the group of Prof. Niemeyer at University of Dortmund, Germany, on Protein-DNA-conjugates. Title of thesis: "Herstellung rekombinanter Proteine für die Synthese von DNA-Protein-Konjugaten". Mark: summa cum laude ("Ausgezeichnet")
- 2004 eight month research assistant at the group of Prof. Sprinzl, University of Bayreuth, Germany
- 2004 Diploma (Biochemistry), Group of Prof. Sprinzl, University of Bayreuth, Germany
- Research semester, Group of Dr. Ubbink, Protein Chemistry (former Metallo Protein Research), University of Leiden, Netherlands: "The Transient Complex of Cytochrome c and Cytochrome f - Investigated by Heteronuclear NMR"
- 1999-2004, Graduate studies in Biochemistry, University of Bayreuth
- 1999 Intermediate Diploma ("Vordiplom")
- 1997-1999, Undergraduate studies in Biochemistry, University of Bayreuth
- 1996/97 Thirteen month of civilian service
- 1996 finished Abitur (final secondary school examination) at the Graf-Anton-Günther-Schule, Oldenburg, Germany
- Synthetic biology
- Chemical Biology
During my stay at Leiden University, where I did some NMR on transient protein complexes, I got into first contact with raytracing. At the moment I am using Swissprot Deepview and Chimera to work with .pdb files and Moray to set up whole scences. For rendering I usually use povray3.6 (esp. to generate .png files with transparent background). For the coverpicture shown below I used a combination of all programs.
I worked a lot with the thermostable P450 CYP119A1, therefore I prepared a little movie for my PhD defence (on the right). In this movie you can see the P450 enzyme CYP119A1 with the inhibitor phenylimidazol bound to the active site. First, the overall fold is shown with the α-helices displayed in blue, the β-sheets in green and turns in grey. Subsequently the van-der-waals surface of the protein is displayed. Then the camera zooms into the active center. You can nicely see that "underneath" the central iron in the heme cofactor there is a cystein as fifth ligand and that the inhibitor occupies the sixth coordinationsite. Afterwards the camera zooms out again and the primary amine groups on the surface of the protein are shown. The movie was prepared from the threedimensional structure 1F4T with the program Chimera and re-coded to the .ogg format using the SUPER Encoder.
- Rabe KS and Niemeyer CM. . pmid:19387449.
- Rabe KS, Spengler M, Erkelenz M, Müller J, Gandubert VJ, Hayen H, and Niemeyer CM. . pmid:19241405.
- Rabe KS, Feldkamp U, and Niemeyer CM. Semisynthetic DNA-protein conjugates for fabrication of nucleic acid based nanostructures. AIP Conf. Proc. 2009; 1062(1) 19-32. doi: 10.1063/1.3012299 Permalink
- Rabe KS, Gandubert VJ, Spengler M, Erkelenz M, and Niemeyer CM. . pmid:18622752.
- Rabe KS, Kiko K, and Niemeyer CM. . pmid:18157853.
- Svahn MG, Rabe KS, Barger G, El-Andaloussi S, Simonson OE, Didier B, Olivier R, Dumy P, Brandén LJ, Niemeyer CM, and Smith CIE. High-Throughput Identification of Combinatorial Ligands for DNA Delivery in Cell Culture. AIP Conf. Proc. 2009; 1062(1) 73-88. doi: 10.1063/1.3012306 Permalink
- Agafonov DE, Rabe KS, Grote M, Voertler CS, and Sprinzl M. . pmid:16444758.
- Agafonov DE, Rabe KS, Grote M, Huang Y, and Sprinzl M. . pmid:15811322.
- Crowley PB, Rabe KS, Worrall JA, Canters GW, and Ubbink M. . pmid:12325008.
SPRINZL MATHIAS (DE); AGAFONOV DMITRY (DE); RABE KERSTEN (DE) ESTERASES FOR MONITORING PROTEIN BIOSYNTHESIS IN VITRO Publication number: WO2006082059
SPRINZL MATHIAS (DE); AGAFONOV DMITRY (DE); RABE KERSTEN (DE) CELL-FREE TRANSLATION SYSTEM FOR THE PRODUCTION OF (CHEMICALLY) MODIFIED PROTEINS Publication number: WO2006082058