User:Kervin D Hilaire/Notebook/Biology 210 at AU

Jan. 21 2015

This is my first Lab journal submission .

KDH _________________________________________________________________________________________________________________________________________________________________________________

Janurary 25, 2015

LAB 1:Biological Life at AU

Lab 1, we examined the organisms of the same species that were prime examples of evolution. We dealt with Chlorophyta, a green algae group. We measured the number of cells of Chlamydomonas, Gonium, and Volvox; and examined other characteristics of the group of organisms.

Cells		Size
Chlamydomonas	125		Isogamous
Gonium		10	Oogamous
Volvox		180	Oogamous

http://openwetware.org/images/a/af/Kh1.png

Transect 2:

Location: American University , near the entrance of the campus right by Hughes and McDowell Hall

http://openwetware.org/images/7/78/Scan.jpeg

http://openwetware.org/images/5/55/FullSizeRender.jpg

Biotic: Tree (3x) Bushes (6x) Mosh Nut Shells Grass

Abiotic: Rock Bench Directional Signs (2) Snow Ice

KH

Great job! Very complete information and well documented. ML

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Janurary 27, 2015

Lab 2: Identify Algae and Protists

Lab 2 focused on using a dichotomous key to identify unknown organisms and also examine algae and protists from our transect. Before all that we brushed up information on Unicellular Eukarya .

http://openwetware.org/images/6/6d/Bio_1.png

Peranema: 20um

Colpidium Sp: 50um

Volvox: 350um

http://openwetware.org/images/5/56/BIO2.png

Brown Algae: 10um

Bursaira Truncatbella: 500um

Chlamydomonans: 5um

Peranema meets the needs of life because it can grow, reproduce, uses oxygen, and responds to its environment.

If the Hay Infusion Culture grew for another two months, the organism that are currently there would multiply and adapt to the new enivromnet , the smell would get stronger. Fungi and bacteria will start to appear. Temperature would affect the community of samples.

http://openwetware.org/images/f/fa/Screen_Shot_2015-01-27_at_10.19.47_PM.png

Serial Dilution Procedure

http://openwetware.org/images/6/69/Screen_Shot_2015-01-27_at_10.36.04_PM.png

KDH

Very good, well organized and documented. Keep up the good work! ML

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February 3, 2015

Lab 3: Microbiology and identifying Bacteria with DNA Sequences

-The strength of the smell has decreased in the Hay Infusion

- The water has become a bit cloudy

-The water also evaporated quit a bit because there is less water than before

The appearance and smell in the Hay Infusion have decreased from week to week because the microorganisms have multiple and adapted to their new environment.

http://openwetware.org/images/c/cd/FullSizeRender-1.jpg

http://openwetware.org/images/0/0f/FullSizeRender-3.jpg

http://openwetware.org/images/6/67/FullSizeRender-13.jpg

The plates without the antibiotic had many colonies appear compared to the plate with antibiotic had one or few appear. The bacteria in the plate is not resistance to tetracycline. There were 3 that were unaffected by the antibiotic.

Tetracycline inhibits protein synthesis by preventing the attachment of aminoacyl-tRNA to the ribosomal acceptor (A) site. Tetracyclines are broad-spectrum agents, exhibiting activity against a wide range of gram-positive and gram-negative bacteria, atypical organisms such as chlamydiae, mycoplasmas, and rickettsiae, and protozoan parasites. (http://www.ncbi.nlm.nih.gov/pmc/articles/PMC99026/)

http://openwetware.org/images/c/c0/IMG_2193.jpg

http://openwetware.org/images/f/fd/IMG_4883.jpeg

KDH

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February 10, 2014

Lab 4: Plantae and Fungi

Purpose

The purpose of this lab was to study the different characteristics of plants and then characterize the plant life living in our transect (transect 2). PCR reactions were ran and sequenced for the genes of the bacteria that were collected the last lab. Lastly, the Burlese funnel was put together to collect invertebrates for lab 4. Invertebrates and the plants will represent our ecosystem

http://openwetware.org/images/9/9e/Screen_Shot_2015-02-11_at_6.25.53_PM.png

Samples 3,4 and 5 reproduce using seeds

http://openwetware.org/images/3/34/Kervvvvv.png

http://openwetware.org/images/b/be/Screen_Shot_2015-02-11_at_6.06.26_PM.png

http://openwetware.org/images/b/b8/Screen_Shot_2015-02-11_at_6.06.59_PM.png

http://openwetware.org/images/f/fb/Screen_Shot_2015-02-11_at_6.06.40_PM.png

http://openwetware.org/images/d/d0/Screen_Shot_2015-02-11_at_6.06.48_PM.png

KDH

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February 17, 2015

Lab 5: Invertebrates

Purpose: Help understand the importance of Invertebrates and learn how simple systems evolved into more complex systems.

Methods:

Berlese Funnel Set up:

1. Pour 25mL of 50:50 ethanol/water Solution into a 50mL conical tube 2. Place a piece of screening material into the bottom of the funnel 3. Then place leaf litter in the top of funnel 4. Next place funnel on to ring stand 5. Place a lighted 40 watt lamp above the funnel, 1-2 inches from the top of the leaf litter 6. Cover everything with foil 7. Leave for a week

Movement of worms: Quick, springing, stationary and almost elastic

Invertebrates:

Bees: 1.5cm Centipede: 30cm Small Crab: 1.5cm Spider: 4cm Millipede: 35cm

Invertebrates found in Transect

http://openwetware.org/images/e/e2/Screen_Shot_2015-02-18_at_3.51.58_PM.png

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February 25, 2015

NCBI blast database

MB7 >MB76-Rev_16S_G01.ab1 NNNNNNNNNGNNNNNNNNNNNNNNNCTCNTGTNACGGTNNNNNACTTCAGGTACCCCAGACTTCCATGGCTTGACGGGCG GTGTGTACAAGGCCCGGGAACGTATTCACCGCGCCATGGCTGATGCGCGATTACTAGCGATTCCAGCTTCATAGAGTCGA GTTGCAGACTCCAATCCGAACTGAGACCGGCTTTCGAGATTCGCATCACATCGCTGTGTAGCTGCCCTCTGTACCGGCCA TTGTATTACGTGTGTGGCCCAAGGCGTAAGGGCCGTGATGATTTGACGTCATCCCCACCTTCCTCTCTACTTGCGTAGGC AGTCTCACTAGAGTCCCCAACTTAATGATGGCAACTAGTGACAGGGGTTGCGCTCGTTGCAGGACTTAACCTAACACCTC ACGGCACGAGCTGACGACAACCATGCAGCACCTTGAAAAATGTCCGAAGAAAAGTCTATTTCTAAACCTGTCATTTCCCA TTTAAGCCTTGGTAAGGTTCCTCGCGTATCATCGAATTAAACCACATAATCCACCGCTTGTGCGGGCCCCCGTCAATTCC TTTGAGTTTCAGACTTGCGTCCGTACTCCCCAGGTGGCTAACTTATCACTTTCGCTTAGTCTCTGAATCCGAAAACCCAA AAACGAGTTAGCATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAGCGT CAGTTGTTGCTTAGTAACCTGCCTTCGCAATTGGTGTTCTAAGTAATATCTATGCATTTCACCGCTACACTACTTATTCC AGCTACTTCAACAACACTCAAGACTTGCAGTATCAATGGCAGTTTCACAGTTAAGCTGTGAGATTTCACCACTGACTTAC AAATCCGCCTACGGACCCTTTAAACCCAATAAATCCCGGATAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACG GGAGTTAGCNNNGCTTANTCGTATAGTACCTTCAGCTAGATACACGTANNTNGGTTTATCCCTATACAAAGAAGTTTANN NCCATNNNNNNNCCTTCANGCNGATGGCTGGATCNNNNNTCACCCATTNNCCAANANTNCNTCACTGCNNN

Chryseobacterium jejeunse: aerobic, Gram-negative, non-motile, yellow-pigmented, straight rod-shaped bacteria

MB8 >MB77-Rev_16S_H01.ab1 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCNNNNNNNNNNANNNTNNNNCTACTTCTGGTAAAACCCGCTCCCATGG TGTGACGGGCGGTGTGTACAAGACCCGGGAACGTATTCACCGCGACATGCTGATCCGCGATTACTAGCGATTCCAACTTC ATGCAGTCGAGTTGCAGACTACAATCCGGACTACGATACACTTTCTGCGATTAGCTCCCCCTCGCGGGTTGGCGGCGCTC TGTATGTACCATTGTATGACGTGTGAAGCCCTACCCATAAGGGCCATGAGGACTTGACGTCATCCCCACCTTCCTCCGGT TTGTCACCGGCAGTCTCATTAGAGTGCCCTTTCGTAGCAACTAATGACAAGGGTTGCGCTCGTTGCGGGACTTAACCCAA CATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTGTACTGGTTCTCTTTCGAGCACTCCCCGATCTCTCGGGG ATTCCAGCCATGTCAAGGGTAGGTAAGGTTTTTCGCGTTGCATCGAATTAATCCACATCATCCACCGCTTGTGCGGGTCC CCGTCAATTCCTTTGAGTTTTAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTGCGTTACCAAGTC AATTAAGACCCGACAACTAGTAGACATCGTTTAGGGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTT CGTGCATGAGCGTCAATCTTGACCCANGGGGCTGCCTTCGCCATCGGTGTTCCTCCACATATCTACGCATTTCACTGCTA CACGTGGAATTCTACCCCCCTCTGCCAGATTCTAGCCTTGCAGTCTCCAATGCAATTCCCAGGTTGAGCCCGGGGATTTC ACATCANACTTACAAAACCGCCNGCGCACGCTTNACGCCCAGTAATTCCGANTAACGCTTGCACCCTACGTATTACCGCG GCTGCTGGCACGTAGTTAGCCNGTGCTTANNNNTCNNNACCGTCATTAGCANAGANATAGCTCTCANCNTTTCNTCCCNG ANAAAGAGCTTTANANCCGAANNNTCTTCANNCNNGCGNATTGNTGGATCAGGNTTTCNC

Janthinobacterium lividum: aerobic, Gram-negative, soil-dwelling, dark-violet bacterium

February 20, 2015t

Purpose

The purpose of this lab was to observe any changes in our zebra fish: One Group one (control) and group two (experimental).

Other observations were made every day. During the time the water was changed and food was given.

Data & Observations

http://openwetware.org/images/e/ea/Unnamed.jpg

'''Contro'''l

Eye size: .242 mm Body length: 1.17 mm (egg) Heart rate: 72 bpm (one visible) Movement: Not moving, but flowing motion visible within eggs Color: Greenish/purple pigment scattered throughout, black eyes

Ethanol

Eye size: .217 mm Body length: 1.103 mm (egg) Heart rate: 98 bpm (one visible) Movement: Not moving, but flowing motion visible within eggs Color: Black/gray coloration, black eyes

http://openwetware.org/images/2/2c/Unnamed-1.jpg

Monday, Feb 23, 2015 5:00pm

'''Control'''

Eye diameter: Avg: 1.35@ 4x Body Length: Avg: 13.5@4x Heart Rate: Moved to fast to calculate Movement: Zipped around/ moved quickly Color Change: No

Ethanol

Eye diameter: Avg: 1.8@4x Body Length: Avg: 13.25@4x Heart Rate: Moved to fast to calculate Movement: No all moved, some remain stable and others zipped around quickly Color Change: No

Monday, March 2, 2015 3:20pm

'''Control'''

Eye diameter: Was unable to see them Body Length: Avg: 16.5@4x Heart Rate:None Movement: None ....did not move :'( Color Change: No ( seemed color less) Dr. B said is was due to Chromatophore The are also being eaten by protists

'''Ethanol'''

Eye diameter: Was unable to see them Body Length: Avg: 17.5@4x Heart Rate: None Movement: None Color Change: No Eaten by protists

'''March 4, 2015'''

http://openwetware.org/images/c/ce/Unnamed-2.jpg