User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/08/29: Difference between revisions
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== | ==Purpose== | ||
* | * To test the absorbance in solutions with various ratio of gold nanoparticles (AU) to BSA (Bovine Serum Albumin) | ||
* To discover trends in the increase of Au/BSA ratios | |||
==Procedure== | |||
* Bovine Serum Albumin stock solution was prepared at 15uM. | |||
* Chloroauric Acid (HAuCl4) stock solution was prepared at 10mM. | |||
* BSA and chloroauric acid at the above concentrations was mixed in various amounts and was diluted with sterile water to the proper ratio. See table below for details. | |||
{| | |||
|- | |||
! Au/BSA ratio | |||
! BSA[uL] | |||
! HAuCl4[uL] | |||
! H2O added[mL] | |||
|- | |||
| 60 | |||
| 253.1 | |||
| 147.9 | |||
| 3.5990 | |||
|- | |||
| 80 | |||
| 253.1 | |||
| 197.2 | |||
| 3.5497 | |||
|- | |||
| 100 | |||
| 253.1 | |||
| 246.6 | |||
| 3.5004 | |||
|- | |||
| 120 | |||
| 253.1 | |||
| 295.9 | |||
| 3.4511 | |||
|- | |||
| 128 | |||
| 253.1 | |||
| 315.6 | |||
| 3.4313 | |||
|- | |||
| 130 | |||
| 253.1 | |||
| 320.5 | |||
| 3.4264 | |||
|- | |||
| 132 | |||
| 253.1 | |||
| 325.4 | |||
| 3.4215 | |||
|- | |||
| 133 | |||
| 253.1 | |||
| 327.9 | |||
| 3.4190 | |||
|- | |||
| 134 | |||
| 253.1 | |||
| 330.4 | |||
| 3.4166 | |||
|- | |||
| 136 | |||
| 253.1 | |||
| 335.3 | |||
| 3.4116 | |||
|- | |||
| 138 | |||
| 253.1 | |||
| 340.2 | |||
| 3.4067 | |||
|- | |||
| 140 | |||
| 253.1 | |||
| 345.2 | |||
| 3.4018 | |||
|- | |||
| 160 | |||
| 253.1 | |||
| 394.5 | |||
| 3.3524 | |||
|- | |||
| 170 | |||
| 253.1 | |||
| 419.1 | |||
| 3.3278 | |||
|} | |||
* 40mL of solution with various Au/BSA ratios were labeled and placed in incubator for four hours at __°C, and left in room temperature until the following week. | |||
==Notes== | |||
* Various ratios of Au/BSA Solutions appeared yellow and transparent when mixed. | |||
* Test tubes with solutions enclosed were wrapped around with aluminum foil to prevent light-stimulated effects. | |||
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__NOTOC__ | __NOTOC__ |
Revision as of 19:16, 2 September 2012
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Purpose
Procedure
Notes
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