Purpose
- To test the absorbance in solutions with various ratio of gold nanoparticles (AU) to BSA (Bovine Serum Albumin)
- To discover trends in the increase of Au/BSA ratios
Procedure
- Bovine Serum Albumin stock solution was prepared at 15uM.
- Chloroauric Acid (HAuCl4) stock solution was prepared at 10mM.
- BSA and chloroauric acid at the above concentrations was mixed in various amounts and was diluted with sterile water to the proper ratio. See table below for details.
| Au/BSA ratio
|
BSA[uL]
|
HAuCl4[uL]
|
H2O added[mL]
|
| 60
|
253.1
|
147.9
|
3.5990
|
| 80
|
253.1
|
197.2
|
3.5497
|
| 100
|
253.1
|
246.6
|
3.5004
|
| 120
|
253.1
|
295.9
|
3.4511
|
| 128
|
253.1
|
315.6
|
3.4313
|
| 130
|
253.1
|
320.5
|
3.4264
|
| 132
|
253.1
|
325.4
|
3.4215
|
| 133
|
253.1
|
327.9
|
3.4190
|
| 134
|
253.1
|
330.4
|
3.4166
|
| 136
|
253.1
|
335.3
|
3.4116
|
| 138
|
253.1
|
340.2
|
3.4067
|
| 140
|
253.1
|
345.2
|
3.4018
|
| 160
|
253.1
|
394.5
|
3.3524
|
| 170
|
253.1
|
419.1
|
3.3278
|
- 40mL of solution with various Au/BSA ratios were labeled and placed in incubator for four hours at __°C, and left in room temperature until the following week.
Notes
- Various ratios of Au/BSA Solutions appeared yellow and transparent when mixed.
- Test tubes with solutions enclosed were wrapped around with aluminum foil to prevent light-stimulated effects.
|
Experimental Biological Chemistry I
