User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/09/04: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
Line 6: Line 6:
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==Entry title==
==Purpose==
* Insert content here...
* To run UV-Vis analysis on Au/BSA solutions at various ratios.  
* To remake solutions that did not give expected results.
* To make Tris Buffer at 100mM at pH 8.0 and 10.0
 
==Procedure==
* The absorbance of each sample...
 
==Notes==
* The original sets of solutions did not form nano-particles, solutions appeared yellow in color. It was predicted that the HAuCl4 reacted with gold specula during the transfer.
* A new set of solutions with different Au/BSA ratios were made beforehand at the same ratio and concentrations. For details of the concentrations, see [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/08/29|entry from 2012/08/29]]
* The new set of solutions with different ratios of Au and BSA appeared purpose in color. The 60 Au/BSA solution appeared cloudy and white. The 80 Au/BSA solution appeared as a purpose homogenous solution. The Au/BSA solution with other ratios are transparent with purpose fiber formations.  




<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
|}
 


__NOTOC__
__NOTOC__

Revision as of 12:55, 4 September 2012

Experimental Biological Chemistry I <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Purpose

  • To run UV-Vis analysis on Au/BSA solutions at various ratios.
  • To remake solutions that did not give expected results.
  • To make Tris Buffer at 100mM at pH 8.0 and 10.0

Procedure

  • The absorbance of each sample...

Notes

  • The original sets of solutions did not form nano-particles, solutions appeared yellow in color. It was predicted that the HAuCl4 reacted with gold specula during the transfer.
  • A new set of solutions with different Au/BSA ratios were made beforehand at the same ratio and concentrations. For details of the concentrations, see entry from 2012/08/29
  • The new set of solutions with different ratios of Au and BSA appeared purpose in color. The 60 Au/BSA solution appeared cloudy and white. The 80 Au/BSA solution appeared as a purpose homogenous solution. The Au/BSA solution with other ratios are transparent with purpose fiber formations.
Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I/2012/09/04&oldid=623505"