User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/09/19: Difference between revisions
From OpenWetWare
Keyun Wang (talk | contribs) No edit summary |
Keyun Wang (talk | contribs) No edit summary |
||
Line 30: | Line 30: | ||
|} | |} | ||
* Samples with varying concentrations of luminol or H2O2 were diluted from those stock solutions. To make 625uM luminol, 50uL of 1.25uM was mixed with 50uL of distilled water. To make 850uM of H2O2, 50uL of 1.7mM H2O2 was mixed with 50uL of distilled water. | * Samples with varying concentrations of luminol or H2O2 were diluted from those stock solutions. To make 625uM luminol, 50uL of 1.25uM was mixed with 50uL of distilled water. To make 850uM of H2O2, 50uL of 1.7mM H2O2 was mixed with 50uL of distilled water. | ||
* Fluorometer was set to wavelength | * Fluorometer was set to wavelength 430nm. All data were collected for a period of ~300 seconds to make sure samples has reached equilibrium and had completed in fluorescing. | ||
==Notes== | ==Notes== |
Revision as of 10:29, 25 September 2012
Experimental Biological Chemistry I | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||
Purpose
Procedure
Notes
|