User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/09/25: Difference between revisions
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==Procedure== | ==Procedure== | ||
* Four LB solution for starter culture was made by mixing 0.875g of LB in 35mL of distilled water in 250mL Erlenmeyer flasks. Four of 1L LB solution for cell amplification 250g with 1L distilled water in 2.8L Erlenmeyer flasks. Broth were autoclaved in liquid cycle. | * Four LB solution for starter culture was made by mixing 0.875g of LB in 35mL of distilled water in 250mL Erlenmeyer flasks. Four of 1L LB solution for cell amplification 250g with 1L distilled water in 2.8L Erlenmeyer flasks. Broth were autoclaved in liquid cycle. | ||
*'''[[User:Abigail E. Miller|Abigail E. Miller]] 17:46, 7 October 2012 (EDT)''': did you put exactly 0.875 g and 250 g (shoudl be 25 g)? what is liquid cycle and how did the autoclave work? | |||
* New horseradish peroxidase were made by mixing 1mg of horseradish peroxidase in 1mL of distilled water. The calculated final concentration of horseradish peroxidase is 2.3uM. The enzyme were diluted ten folds to 230nM and 23nM to test luminol and H2O2 reaction. | * New horseradish peroxidase were made by mixing 1mg of horseradish peroxidase in 1mL of distilled water. The calculated final concentration of horseradish peroxidase is 2.3uM. The enzyme were diluted ten folds to 230nM and 23nM to test luminol and H2O2 reaction. | ||
* New 4-Iodophenol was made with water and sodium bicarbonate. See [[User:Melissa Novy/Notebook/CHEM-571/2012/09/25|Melissa's Notebook]] for details on making the 4-Iodophenol stock solution. | * New 4-Iodophenol was made with water and sodium bicarbonate. See [[User:Melissa Novy/Notebook/CHEM-571/2012/09/25|Melissa's Notebook]] for details on making the 4-Iodophenol stock solution. | ||
* One colony from ADA transformation cell plate were taken and dipped into 4mL starter LB culture. 4uL of 0.4M ampicillin were added to starter. Cell culture were placed in shaker overnight and for 15 hours overnight under 37C. | * One colony from ADA transformation cell plate were taken and dipped into 4mL starter LB culture. 4uL of 0.4M ampicillin were added to starter. Cell culture were placed in shaker overnight and for 15 hours overnight under 37C. | ||
*'''[[User:Abigail E. Miller|Abigail E. Miller]] 17:46, 7 October 2012 (EDT)''':you did not use ampicillin you used kanamycin. what speed did it shake at? | |||
==Notes== | ==Notes== |
Revision as of 14:46, 7 October 2012
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