User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/09/26: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Experimental Biological Chemistry I</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Experimental Biological Chemistry I</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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* Elution and binding buffers were made. See [[User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/09/26|Dhea's Notebook]] for the details in making elution and binding buffers used for future cell resuspension and protein purification. | * Elution and binding buffers were made. See [[User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/09/26|Dhea's Notebook]] for the details in making elution and binding buffers used for future cell resuspension and protein purification. | ||
* After 4 hours in the shaker, cells were transferred into centrifuge bottles with mass ~1 grams apart. Cells were centrifuged at 4500rpm for 15 minutes at 4C. Supernatant were discarded, pellet were resuspended with 30mL of binding buffer. Cell resuspension were stored in -80C until next week. This process is repeated two times for more cells. | * After 4 hours in the shaker, cells were transferred into centrifuge bottles with mass ~1 grams apart. Cells were centrifuged at 4500rpm for 15 minutes at 4C. Supernatant were discarded, pellet were resuspended with 30mL of binding buffer. Cell resuspension were stored in -80C until next week. This process is repeated two times for more cells. | ||
* Luminol-peroxide reaction were further experimented. Luminol concentration were altered, and concentration of horseradish peroxidase were altered. For details of the | * Luminol-peroxide reaction were further experimented. Luminol concentration were altered, and concentration of horseradish peroxidase were altered. For details of the experiment, and details of data obtained, see [[User:Melissa Novy/Notebook/CHEM-571/2012/09/26|Melissa's Notebook]] for information on concentration of luminol, H2O2, and HRP assay used in each trial. For initial and final concentration of the solutions, see below table: | ||
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Latest revision as of 22:05, 26 September 2017
Experimental Biological Chemistry I | Main project page Previous entry Next entry | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Purpose
Procedure
Notes
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