User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/10/03: Difference between revisions
From OpenWetWare
(fix raw html notebook nav) |
|||
(2 intermediate revisions by one other user not shown) | |||
Line 2: | Line 2: | ||
|- | |- | ||
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Experimental Biological Chemistry I</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Experimental Biological Chemistry I</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
|- | |- | ||
| colspan="2"| | | colspan="2"| | ||
Line 11: | Line 11: | ||
==Procedure== | ==Procedure== | ||
* Column from | * Column from purifier were washed with 100mL binding buffer at pH 7.5 with 500nM Tris, 200nM NaCl, and 30mM of imidazol. | ||
* Protein supernatant filtered from yesterday was injected into column. | * Protein supernatant filtered from yesterday was injected into column. | ||
* Protein supernatant were run through the column with AKTA purifier. Flow through were collected in 50ml falcon tube just in case protein failed to bind to column. | * Protein supernatant were run through the column with AKTA purifier. Flow through were collected in 50ml falcon tube just in case protein failed to bind to column. | ||
Line 18: | Line 17: | ||
* UV-vis showed concentration protein elution at 280nm absorptivity, and indicated the elution test tube with most concentrated ADA protein. | * UV-vis showed concentration protein elution at 280nm absorptivity, and indicated the elution test tube with most concentrated ADA protein. | ||
* Two test tubes of ADA protein were collected in a 15mL falcon tube, and flow through of the protein are stored in 4C. | * Two test tubes of ADA protein were collected in a 15mL falcon tube, and flow through of the protein are stored in 4C. | ||
* For specific parameter for the column purifier, please refer to [[User:Melissa Novy/Notebook/CHEM-571/2012/10/03|Melissa's Notebook]] for the details of the column purifier and conditions which the ADA protein was run at. | |||
* | |||
==Discussion== | ==Discussion== |
Latest revision as of 22:05, 26 September 2017
Experimental Biological Chemistry I | Main project page Previous entry Next entry |
Purpose
Procedure
Discussion
|