User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/10/17: Difference between revisions
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==Results== | ==Results== | ||
* Gel | * Gel was not observed under UV due to the fact that the ladder used was not the right size. Resulting gel shown a smear of DNA ladder about 3cm, and a concentration of dye at the wells of DNA gel, indicating that the gel did not work properly. Picture of gel was not taken. | ||
* The atomic absorbance spectrometer were run on samples, tables is shown below for standard calibration and concentration of gold measured. | * The atomic absorbance spectrometer were run on samples, tables is shown below for standard calibration and concentration of gold measured. | ||
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| 40||-||0.7752||0.0126 | | 40||-||0.7752||0.0126 | ||
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Table I: Standard Calibration | |||
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| align="center" style="background:#f0f0f0;"|'''Samples''' | | align="center" style="background:#f0f0f0;"|'''Samples''' | ||
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| 170||-1.469||0.0078||0.0067 | | 170||-1.469||0.0078||0.0067 | ||
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Table II: Absorbance for gold in Au/BSA solutions made from [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/10/16|2012/10/16]] | |||
* Calibration curve was created from standard solutions listed in the first table. Please refer to [[User:Melissa Novy/Notebook/CHEM-571/2012/10/17|Melissa's Notebook]] for graphical representation of the curve. The slope was determine to be 0.0214 abs/ppm. This rate is going to be used to calculate the concentration of gold in Au/BSA fibers. The absorbance of each Au/BSA sample was shown in above table II. | |||
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Revision as of 13:12, 26 October 2012
Experimental Biological Chemistry I | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Purpose
Procedure
DNA ladder --> E34K --> E34A --> E34A --> K110A --> K110A --> E34K
2000rpm for 5 minutes, 2000rpm for 10 minutes, 2500rpm for 5 minutes, 3000rpm for 10 minutes, 4700rpm for 15 minutes, 4700rpm for 20 minutes
5ppm, 8ppm, 10ppm, 15ppm, 20ppm, 25ppm, 30ppm, 40ppm
Notes
Results
Table I: Standard Calibration
Table II: Absorbance for gold in Au/BSA solutions made from 2012/10/16
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