User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/10/17: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Experimental Biological Chemistry I</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Experimental Biological Chemistry I</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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| colspan="2"| | | colspan="2"| | ||
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4700rpm for 15 minutes, 4700rpm for 20 minutes | 4700rpm for 15 minutes, 4700rpm for 20 minutes | ||
* Some fibers in some solutions still remained on top of solution. The supernatant were taken out using a pipet and placed in 15mL falcon tube. | * Some fibers in some solutions still remained on top of solution. The supernatant were taken out using a pipet and placed in 15mL falcon tube. | ||
* Supernatant with different ratios of Au/BSA were run under atomic absorbance spectrophotometer. | * Supernatant with different ratios of Au/BSA were run under atomic absorbance spectrophotometer. Blank was done with HCl. Wavelength is excited at 242.8nm. 8 standards were run at the following concentration: | ||
5ppm, 8ppm, 10ppm, 15ppm, 20ppm, 25ppm, 30ppm, 40ppm | |||
* 12 samples of Au/BSA with various ratios were run in atomic absorbance spectrophotometer after standards were taken. | |||
==Notes== | ==Notes== | ||
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==Results== | ==Results== | ||
* | * Gel was not observed under UV due to the fact that the ladder used was not the right size. Resulting gel shown a smear of DNA ladder about 3cm, and a concentration of dye at the wells of DNA gel, indicating that the gel did not work properly. Picture of gel was not taken. | ||
* The atomic absorbance spectrometer were run on samples, tables is shown below for standard calibration and concentration of gold measured. | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''standard''' | |||
| align="center" style="background:#f0f0f0;"|'''Concentration''' | |||
| align="center" style="background:#f0f0f0;"|'''absorbance''' | |||
| align="center" style="background:#f0f0f0;"|'''background''' | |||
|- | |||
| blank||||-0.1347||0.0015 | |||
|- | |||
| 5||-||0.1152||0.002 | |||
|- | |||
| 8||-||0.1795||0.0057 | |||
|- | |||
| 10||-||0.2326||0.0098 | |||
|- | |||
| 15||-||0.3279||0.0085 | |||
|- | |||
| 20||-||0.4342||0.0092 | |||
|- | |||
| 25||-||0.5281||0.0103 | |||
|- | |||
| 30||-||0.6326||0.0125 | |||
|- | |||
| 40||-||0.7752||0.0126 | |||
|} | |||
Table I: Standard Calibration | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Samples''' | |||
| align="center" style="background:#f0f0f0;"|'''Concentration''' | |||
| align="center" style="background:#f0f0f0;"|'''absorbance''' | |||
| align="center" style="background:#f0f0f0;"|'''background''' | |||
|- | |||
| 80||32.3627||0.6573||0.0154 | |||
|- | |||
| 100||33.5087||0.6793||0.0112 | |||
|- | |||
| 120||32.1596||0.6534||0.0137 | |||
|- | |||
| 128||23.4034||0.4853||0.0145 | |||
|- | |||
| 130||22.096||0.4602||0.0138 | |||
|- | |||
| 132||32.0606||0.6515||0.0157 | |||
|- | |||
| 133||8.9596||0.208||0.0128 | |||
|- | |||
| 134||-1.4429||0.0083||0.0091 | |||
|- | |||
| 136||-0.198||0.0322||0.0075 | |||
|- | |||
| 138||-1.7398||0.0026||0.006 | |||
|- | |||
| 160||-2.0315||-0.003||0.0101 | |||
|- | |||
| 170||-1.469||0.0078||0.0067 | |||
|} | |||
Table II: Absorbance for gold in Au/BSA solutions made from [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/10/16|2012/10/16]] | |||
* Calibration curve was created from standard solutions listed in the first table. Please refer to [[User:Melissa Novy/Notebook/CHEM-571/2012/10/17|Melissa's Notebook]] for graphical representation of the curve. The slope was determine to be 0.0214 abs/ppm. This rate is going to be used to calculate the concentration of gold in Au/BSA fibers. The absorbance of each Au/BSA sample was shown in above table II. | |||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | ||
|} | |} | ||
__NOTOC__ | __NOTOC__ |
Latest revision as of 22:07, 26 September 2017
Experimental Biological Chemistry I | Main project page Previous entry Next entry | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Purpose
Procedure
DNA ladder --> E34K --> E34A --> E34A --> K110A --> K110A --> E34K
2000rpm for 5 minutes, 2000rpm for 10 minutes, 2500rpm for 5 minutes, 3000rpm for 10 minutes, 4700rpm for 15 minutes, 4700rpm for 20 minutes
5ppm, 8ppm, 10ppm, 15ppm, 20ppm, 25ppm, 30ppm, 40ppm
Notes
Results
Table I: Standard Calibration
Table II: Absorbance for gold in Au/BSA solutions made from 2012/10/16
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