User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/10/31

Purpose

• Au/Lysozyme samples were made with the same procedure used to make Au/BSA solutions.

Procedure

• Lysozyme in powder form from chicken egg white obtained from Signma® is used to make the lysozyme stock solution.
• 5mL of 70μM lysozyme stock solution was made in the following way:
  • 0.005g of lysozyme was weighted out
  • 0.005g of lysozyme was transferred to a 15ml falcon tube and mixed well with 5mL of distilled water.
  • See Melissa's Notebook for calculation that stated that 0.005g of lysozyme was needed for 5mL of 70μM of lysozyme stock solution.
• The HAuCl₄ used to make Au/Lysozyme samples was already made on 2012/09/05, and the concentration of the HAuCl₄ was 10mM.
• Au/Lysozyme samples was made with mole ratios listed below:

  20 - 30 - 40 - 50 - 60 - 70 - 80 - 100 - 120 - 130

• Based off the calculations for Au/BSA samples, a few changes were made to calculate the amount of lysozyme and HAuCl₄ needed for Au/Lysozyme samples.
• The table below listed the initial conditions for both lysozyme and HAuCl₄ both theoretically and actually:

• With the above calculation, the actual concentration of lysozyme came out to be 70μM and the actual concentration of HAuCl₄ came out to be 10.501mM. The two concentration was used to calculate the amount of water, lysozyme, HAuCl₄ needed to make the ratios listed above.
• The table below listed the amount of water, lysozyme, HAuCl₄ used for each Au/Lysozyme sample:

• The stock solutions were added with pipetman and non-sterile pipette tips. All samples were made in 10mL large test tubes. All samples were capped and wrapped in aluminum foil.
• All samples were placed in incubator at 85°C for four hours then in room temperature to cool down.