User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/10/31: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Experimental Biological Chemistry I</span> | ||
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | ||
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== | ==Purpose== | ||
* | * Au/Lysozyme samples were made with the same procedure used to make Au/BSA solutions. | ||
==Procedure== | |||
* Lysozyme in powder form obtained from ___company is used to make the lysozyme stock solution. | |||
* 5mL of 70μM lysozyme stock solution was made in the following way: | |||
** 0.005g of lysozyme was weighted out | |||
** 0.005g of lysozyme was transferred to a 15ml falcon tube and mixed well with 5mL of distilled water. | |||
** See [[User:Melissa Novy/Notebook/CHEM-571/2012/10/31|Melissa's Notebook]] for calculation that stated that 0.005g of lysozyme was needed for 5mL of 70μM of lysozyme stock solution. | |||
* The HAuCl<sub>4</sub> used to make Au/Lysozyme samples was already made on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/09/05|2012/09/05]], and the concentration of the HAuCl<sub>4</sub> was 10mM. | |||
* Au/Lysozyme samples was made with mole ratios listed below: | |||
20 - 30 - 40 - 50 - 60 - 70 - 80 - 100 - 120 - 130 | |||
* Based off the calculations for Au/BSA samples, a few changes were made to calculate the amount of lysozyme and HAuCl<sub>4</sub> needed for Au/Lysozyme samples. | |||
* The table below listed the initial conditions for both lysozyme and HAuCl<sub>4</sub> both theoretically and actually: | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''-''' | |||
| align="center" style="background:#f0f0f0;"|'''Lysozyme''' | |||
| align="center" style="background:#f0f0f0;"|'''HAuCl<sub>4</sub>''' | |||
|- | |||
| Molecular Weight [g/mol]||14300||339.79 | |||
|- | |||
| Theoretical Concentration [M]||7.00E-05||0.01 | |||
|- | |||
| Volume of Water to add [L]||0.005||0.025 | |||
|- | |||
| Moles Present [mol]||3.50E-07||0.00025 | |||
|- | |||
| Theoretical Mass Needed [g]||0.005||0.0849 | |||
|- | |||
| Actual Mass Weighted [g]||0.005||0.0892 | |||
|- | |||
| Actual Concentration [M]||0.0007||10501 | |||
|} | |||
* With the above calculation, the actual concentration of lysozyme came out to be 70μM and the actual concentration of HAuCl<sub>4</sub> came out to be 10.501mM. The two concentration was used to calculate the amount of water, lysozyme, HAuCl<sub>4</sub> needed to make the ratios listed above. | |||
* The table below listed the amount of water, lysozyme, HAuCl<sub>4</sub> used for each Au/Lysozyme sample: | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Au/Lysozyme ratio''' | |||
| align="center" style="background:#f0f0f0;"|'''Lysozyme added [uL]''' | |||
| align="center" style="background:#f0f0f0;"|'''HAuCl4 added [uL]''' | |||
| align="center" style="background:#f0f0f0;"|'''Water added [uL]''' | |||
|- | |||
| 20||114.3||15.2||7870.5 | |||
|- | |||
| 30||114.3||22.9||7862.8 | |||
|- | |||
| 40||114.3||30.5||7855.2 | |||
|- | |||
| 50||114.3||38.1||7847.6 | |||
|- | |||
| 60||114.3||45.7||7840 | |||
|- | |||
| 70||114.3||53.3||7832.4 | |||
|- | |||
| 80||114.3||60.9||7824.8 | |||
|- | |||
| 100||114.3||76.2||7809.5 | |||
|- | |||
| 120||114.3||91.4||7794.3 | |||
|- | |||
| 130||114.3||99||7786.7 | |||
|} | |||
* The stock solutions were added with pipetman and non-sterile pipette tips. All samples were made in 10mL large test tubes. All samples were capped and wrapped in aluminum foil. | |||
* All samples were placed in incubator at 85°C for four hours then in room temperature to cool down. | |||
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Revision as of 21:23, 23 November 2012
Experimental Biological Chemistry I | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Purpose
Procedure
20 - 30 - 40 - 50 - 60 - 70 - 80 - 100 - 120 - 130
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