User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/11/04: Difference between revisions
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* After centrifugation, pellets and supernatants form. The supernatants were discarded. The four pellets were resuspended with resuspending 4mL of autoclaved LB media in first pellet, then in the second pellet, third pellet, then fourth pellet. After 4 pellets from 4 different 50mL falcon tubes were resuspended, 1mL of resuspension were placed into each 1L autoclaved LB media from [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/11/03|11/03/2012]]. | * After centrifugation, pellets and supernatants form. The supernatants were discarded. The four pellets were resuspended with resuspending 4mL of autoclaved LB media in first pellet, then in the second pellet, third pellet, then fourth pellet. After 4 pellets from 4 different 50mL falcon tubes were resuspended, 1mL of resuspension were placed into each 1L autoclaved LB media from [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/11/03|11/03/2012]]. | ||
* Four aliquots of IPTG were added into each Fernbach flasks with cell culture before loaded into orbital shaker. | * Four aliquots of IPTG were added into each Fernbach flasks with cell culture before loaded into orbital shaker. | ||
* Cultures were left in orbital shaker for 4 hours, | * Cultures were left in orbital shaker for 4 hours, 210rpm, under 37°C. | ||
* After 4 hours of shaking, 1mL of cell were placed into a cuvette and run under UV-vis to test cell growth. The absorbance of cells in four Fernbach flasks were as follows: | * After 4 hours of shaking, 1mL of cell were placed into a cuvette and run under UV-vis to test cell growth. The absorbance of cells in four Fernbach flasks were as follows: | ||
Revision as of 13:06, 4 November 2012
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Purpose
Procedure
Molecular weight: 238.31g/mol 0.4M × 0.004L = 0.0016mol of IPTG needed 238.31g/mol × 0.0016mol = 0.3813g of IPTG needed
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