User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/11/13: Difference between revisions
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* The absorbance obtained were graphed with absorption versus concentration of HCl. The slope of the plot was used to calculate the concentration of gold in Au/HRP and Au/lysozyme solutions. Because the absorption data and graph were not saved during experimental process, the results cannot be displayed in Data section. | * The absorbance obtained were graphed with absorption versus concentration of HCl. The slope of the plot was used to calculate the concentration of gold in Au/HRP and Au/lysozyme solutions. Because the absorption data and graph were not saved during experimental process, the results cannot be displayed in Data section. | ||
* Supernatant of Au/HRP samples made on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/11/07|2012/11/07]] with a ratio ranging from 10 to 450 were run using Atomic Absorption Spectrometry. | * Supernatant of Au/HRP samples made on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/11/07|2012/11/07]] with a ratio ranging from 10 to 450 were run using Atomic Absorption Spectrometry. | ||
*Supernatant of Au/lysozyme samples made on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/10/31|2012/10/31]] with a ratio ranging from 20 to 130 were also run using Atomic Absorption Spectrometry. | * Supernatant of Au/lysozyme samples made on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/10/31|2012/10/31]] with a ratio ranging from 20 to 130 were also run using Atomic Absorption Spectrometry. | ||
* Distilled water was prepared in a 280mL flask to rinse off samples after Au/HRP and Au/lysozyme samples were run. | |||
* Samples with different ratios of gold to either HRP or lysozyme was run by inserting sample tube into the supernatant of samples. The purple fibers in the samples were avoided to prevent clogging. After the absorbance of samples were taken, the sample tubes were inserted into distilled water for rinsing. This process was repeated until all samples were run. | |||
* The absorbance for each Au/HRP and Au/lysozyme samples were collected by the atomic absorbance spectrometer. The absorbance for each sample were fitted into the slope of calibration curve to obtain the concentration of gold in unit of ppm. Because the raw data were lost, the numbers cannot be shown here. | |||
==Data on Atomic Absorption Spectrometry of Au/ADA and Au/HRP solutions== | ==Data on Atomic Absorption Spectrometry of Au/ADA and Au/HRP solutions== |
Revision as of 16:21, 23 November 2012
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Purpose
Procedure on UV-vis spectrometry of Au/HRP
10 - 50 - 100 - 150 - 200 - 250 - 300 - 350 - 400 - 450
Data on UV-vis spectrometry of Au/HRP
Procedure on Atomic Absorption Spectrometry of Au/lysozyme and Au/HRP solutions
5ppm - 8ppm - 10ppm - 15ppm - 20ppm - 25ppm - 30ppm - 40ppm
Data on Atomic Absorption Spectrometry of Au/ADA and Au/HRP solutions |