User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/11/13: Difference between revisions
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==Data on Atomic Absorption Spectrometry of Au/ADA and Au/HRP solutions== | ==Data on Atomic Absorption Spectrometry of Au/ADA and Au/HRP solutions== | ||
* The concentration of gold in unit of ppm was calculated by converting absorbance into concentration using the HCl calibration curve. | |||
* The concentration of gold for corresponding ratios of Au/HRP and Au/Lysozyme were displayed in table below: | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Au/HRP ratio''' | |||
| align="center" style="background:#f0f0f0;"|'''Concentration of Au[ppm]''' | |||
| align="center" style="background:#f0f0f0;"|'''Au/Lysozyme Ratio''' | |||
| align="center" style="background:#f0f0f0;"|'''Concentration of Au[ppm]''' | |||
|- | |||
| 10||-1.5443||20||0.0057 | |||
|- | |||
| 50||1.8191||30||1.9388 | |||
|- | |||
| 100||11.7714||40||2.7586 | |||
|- | |||
| 150||13.1419||50||3.806 | |||
|- | |||
| 200||23.6748||60||4.5002 | |||
|- | |||
| 250||24.4588||70||-2.4599 | |||
|- | |||
| 300||27.876||80||-2.4839 | |||
|- | |||
| 350||25.4761||100||-2.5856 | |||
|- | |||
| 400||35.1173||120||-2.6394 | |||
|- | |||
| 450||25.6198||130||-2.6454 | |||
|} | |} | ||
* A graph was made with concentration of gold in ppm in supernatant versus mole ratio of Au to HRP and displayed below: | |||
[[Image:AAS_AuHRP.JPG|600px]] | |||
* From the graph above, increase in mole ratio of Au/HRP leads to an linear trend of increase in gold concentration in supernatant. This indicates no protein aggregation would form as mole ratio of Au/HRP increases. The result provide a contrast with results from [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/10/17|2012/10/17]] with the maximum concentration of gold in supernatant at low mole ratios of Au/BSA and minimum concentration of gold in supernatant once the mole ratios of Au/BSA reaches over 134. | |||
* The result indicate that protein aggregation is less likely to form when HRP is used to replace BSA. This might be caused by the nature of HRP compare to BSA and different behaviors when proteins unfold and refold. | |||
* Gold concentrations in Au/Lysozyme samples were also determined and plotted onto the graph "Concentration of Au in Solution of Au-Lysozyme" below: | |||
[[Image:AAS_AuLys.JPG|600px]] | |||
* |
Revision as of 20:20, 23 November 2012
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Purpose
Procedure on UV-vis spectrometry of Au/HRP
10 - 50 - 100 - 150 - 200 - 250 - 300 - 350 - 400 - 450
Data on UV-vis spectrometry of Au/HRP
Procedure on Atomic Absorption Spectrometry of Au/lysozyme and Au/HRP solutions
5ppm - 8ppm - 10ppm - 15ppm - 20ppm - 25ppm - 30ppm - 40ppm
Data on Atomic Absorption Spectrometry of Au/ADA and Au/HRP solutions
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