User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/11/27: Difference between revisions
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* This process was repeated for all three protein fractions: ADA fraction 1&3 made on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/11/06|2012/11/06]], ADA fraction 2 made on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/11/06|2012/11/06]], and ADA fraction purified on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/10/03|2012/10/03]]. | * This process was repeated for all three protein fractions: ADA fraction 1&3 made on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/11/06|2012/11/06]], ADA fraction 2 made on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/11/06|2012/11/06]], and ADA fraction purified on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/10/03|2012/10/03]]. | ||
* ADA fractions in 15mL falcon tubes were stored in 4°C refrigerator. | * ADA fractions in 15mL falcon tubes were stored in 4°C refrigerator. | ||
==Notes for Dialysis== | |||
* It was observed that white precipitants were found at the bottom of dialysis tubing, and suspected that due to a lack of salt in solution with ADA proteins, the ADA proteins falls out of solution. This could predict a potential difference in UV-vis and Atomic Absorption spectrometer results in finalized product of Au/ADA samples made with dialyzed ADA compare to Au/ADA samples made on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/11/14|2012/11/14]]. | |||
==Procedure for making dialyzed Au/ADA samples== | ==Procedure for making dialyzed Au/ADA samples== |
Revision as of 19:49, 27 November 2012
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Purpose
Procedure for Dialysis
Notes for Dialysis
Procedure for making dialyzed Au/ADA samples
60 - 70 - 80 - 90 - 100 - 110 - 120 - 130 - 140 - 150
Procedure for Running UV-vis Spectrometer on Au/ADA and Au/HRP samples
Results for Running UV-vis Spectrometer on Au/ADA and Au/HRP samples |