User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/11/27: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Experimental Biological Chemistry I</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Experimental Biological Chemistry I</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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| 500mM at pH 10||150 Au/HRP||1mL | | 500mM at pH 10||150 Au/HRP||1mL | ||
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* After resuspension, the solution with added buffer was left in room temperature. The resuspension was checked after 24 hours for possibilities of fiber precipitation. | * The 1mL tris buffer were inserted into samples and pipetted up and down to mix until no fibers were observed. | ||
* After resuspension, the solution with added buffer was left in room temperature. The resuspension was checked after 24 hours for possibilities of fiber precipitation. | |||
==Results for Fiber resuspension in Tris buffer== | ==Results for Fiber resuspension in Tris buffer== | ||
* The fibers were resuspended fully after pipetting solution up and down to mix. A picture of resuspended solution is shown below: | * The fibers were resuspended fully after pipetting solution up and down to mix. A picture of resuspended solution is shown below: | ||
[[ | [[Image:Photo13.jpg|400px]] | ||
* After 24 hours, no purple fibers were formed, thus it can be concluded that the addition of salt into solution interacts with gold nanoparticle aggregation in a way that disrupts the aggregation. The lack of precipitation after 24 hours can be due to the gold nanoparticles being more stable when interacting with Tris buffer. | * After 24 hours, no purple fibers were formed, thus it can be concluded that the addition of salt into solution interacts with gold nanoparticle aggregation in a way that disrupts the aggregation. The lack of precipitation after 24 hours can be due to the gold nanoparticles being more stable when interacting with Tris buffer. | ||
* A purple homogenous solution was yielded after the addition of Tris buffers at all three concentrations used. Thus, it can be safe to conclude that Tris buffer at pH 10.0 can successfully resuspend fibers at a 6:1 ratio of solution to tris buffer. | * A purple homogenous solution was yielded after the addition of Tris buffers at all three concentrations used. Thus, it can be safe to conclude that Tris buffer at pH 10.0 can successfully resuspend fibers at a 6:1 ratio of solution to tris buffer. | ||
* Although the exact mechanism for resuspension is unknown, successful resuspension might be explained with following reasoning: | * Although the exact mechanism for resuspension is unknown, successful resuspension might be explained with following reasoning: | ||
** | ** The presence of salt into the solution disrupts the protein aggregation and stabilizes the disrupted gold nanoparticles with surrounding salts in solution. | ||
** The isoelectric point for the protein aggregation might cause the aggregation to interact with the positive and negative charge of the salt in solution. After aggregation is disrupted, pH 10 of the protein allowed gold nanoparticles to be stable in solution. | |||
Latest revision as of 22:17, 26 September 2017
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Purpose
Procedure for Dialysis
Notes for Dialysis
Procedure for making dialyzed Au/ADA samples
60 - 70 - 80 - 90 - 100 - 110 - 120 - 130 - 140 - 150
Procedure for Running UV-vis Spectrometer on Au/ADA and Au/HRP samples
Results for Running UV-vis Spectrometer on Au/ADA and Au/HRP samples
Procedure for Running AA Spectrometer on Au/ADA and Au/HRP samples
5ppm - 8ppm - 10ppm - 15ppm - 20ppm - 25ppm - 30ppm - 40ppm
Results for Running AA Spectrometer on Au/ADA and Au/HRP samples
Procedure for Fiber resuspension in Tris buffer
Results for Fiber resuspension in Tris buffer
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