User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2012/11/28: Difference between revisions
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==Results for Running Au/ADA samples on Atomic Absorption Spectrometer== | ==Results for Running Au/ADA samples on Atomic Absorption Spectrometer== | ||
* The HCl with gold for calibration made up a calibration curve for establishing the concentration of Au/ADA samples. The absorbance measured for HCl with gold were listed in the following table: | * The HCl with gold for calibration made up a calibration curve for establishing the concentration of Au/ADA samples. The absorbance measured for HCl with gold were listed in the following table: | ||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''HCl with gold[ppm]''' | |||
| align="center" style="background:#f0f0f0;"|'''Absorbance''' | |||
|- | |||
| 5||0.1125 | |||
|- | |||
| 8||0.1604 | |||
|- | |||
| 10||0.2012 | |||
|- | |||
| 15||0.278 | |||
|- | |||
| 20||0.358 | |||
|- | |||
| 25||0.4342 | |||
|- | |||
| 30||0.5077 | |||
|- | |||
| 40||0.6435 | |||
|} | |||
* A graph plotting HCl to gold in parts per million versus absorbance is shown below: | |||
[[Image:Absorbance gold HCl ppm.png|350px]] | |||
* The equation on graph was used to calculate the concentration of gold in Au/ADA samples. The following equation was used: | |||
Absorbance = 0.0153 * Concentration + 0.045 | |||
* Absorbance for all Au/ADA samples were taken. Below is a table listing the absorbance of each Au/ADA samples. Concentration of gold in parts per million is also listed after converting from absorbance to concentration using the formula above: | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Au/ADA mole ratios''' | |||
| align="center" style="background:#f0f0f0;"|'''Absorbance(Abs.)''' | |||
| align="center" style="background:#f0f0f0;"|'''Concentration[ppm]''' | |||
|- | |||
| 60||0.0657||1.3572 | |||
|- | |||
| 70||0.041||-0.2608 | |||
|- | |||
| 80||0.1011||3.6762 | |||
|- | |||
| 90||0.0292||-1.0337 | |||
|- | |||
| 100||0.0246||-1.331 | |||
|- | |||
| 110||0.0259||-1.2499 | |||
|- | |||
| 120||0.0246||-1.3351 | |||
|- | |||
| 130||0.0222||-1.4923 | |||
|- | |||
| 140||0.0264||-1.2172 | |||
|- | |||
| 150||0.0229||-1.4464 | |||
|} | |||
* A graph was made with concentration in parts per million of gold versus the Au/ADA samples in mole ratios. | |||
[[Image:Concentration versus Au ADA mole ratios.png|350px]] | |||
* From the graph above, it can be seen that the concentration calculated were below zero. This can indicate that the spectrometer was not properly calibrated. Furthermore, a reference sample with water was not run for this set of data, thus the data cannot be readjusted. Despite the negative concentration, it can be seen that the gold concentration in Au/ADA samples remain relatively the same. | |||
* There was a high peak at 80 Au/ADA, but it was due to the accidental intake of fibers instead of solutions. With the data point at 80Au/ADA excluded, it can be concluded that there is no relationship between mole ratio of Au/ADA and gold concentration in solutions. | |||
==Procedure for Au/ADA Resuspension in Tris Buffer== | ==Procedure for Au/ADA Resuspension in Tris Buffer== | ||
==Results for Au/ADA Resuspension in Tris Buffer== | ==Results for Au/ADA Resuspension in Tris Buffer== |
Revision as of 20:43, 30 November 2012
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Purpose
Procedure for Running Au/ADA samples on UV-vis Spectrophotometer
60-70-80-90-100-110-120-130-140-150
Results for Running Au/ADA samples on UV-vis Spectrophotometer
[Insert Picture]
Procedure for Running Au/ADA samples on Atomic Absorption Spectrometer
5-8-10-15-20-25-30-40
Results for Running Au/ADA samples on Atomic Absorption Spectrometer
Absorbance = 0.0153 * Concentration + 0.045
Procedure for Au/ADA Resuspension in Tris BufferResults for Au/ADA Resuspension in Tris Buffer |