User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/01/30

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(Autocreate 2013/01/30 Entry for User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I)
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Experimental Biological Chemistry II</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Entry title==
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==Purpose==
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* Insert content here...
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* The purpose today is to autoclave LB medium to prepare for cell culture.
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* Also, Ag+ incorporated clay is going to be filtered with water and ethanol to isolate clay from water/ethanol solution.
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==Procedure==
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* 50mL LB media was made using the following way:
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  0.50g Bacto-Trypsin
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  0.25g Bacto-Yeast Extract
 +
  0.50g Sodium Chloride
 +
  50mL of distilled water
 +
  Note: LB media obtained from Genova® with 2:1:2 proportion was used instead. 1.25g of LB powder was weighted out.
 +
* Each of the 50mL LB media was placed in two 125mL glass bottles and four 250mL Erlenmeyer flask.
 +
* 500mL of Agar was made using the following in a 1000mL Erlenmeyer flask:
 +
  12.5g of LB media powder
 +
  500mL of distilled water
 +
  7.5g of Trypsin Soy Agar
 +
* The Erlenmeyer flask obtaining LB media or Agar were covered with aluminum foil, the bottles containing LB were capped loosely. All media were autoclaved under liquid cycle.
 +
* The clay+AgNO<sub>3</sub> mixture was taken off hot plate.
 +
* A filter funnel, attached to 500mL Erlenmeyer flask then attached to a pump, is covered with a paper filter. Surface of paper filter was wetted and attached onto filter funnel through adding water.
 +
* The clay+AgNO<sub>3</sub> mixture was then transfered into the filter funnel with suction applied.
 +
* Aluminum foil was used to cover the funnel to avoid silver to interact with light.
 +
 
 +
==Results==
 +
* The clay+AgNO<sub>3</sub> mixture when taken off the hot plate appeared black. However, during filtration, the filtered liquid solution remained clear in color.

Revision as of 14:00, 30 January 2013

Experimental Biological Chemistry II Main project page
Previous entry      Next entry

Purpose

  • The purpose today is to autoclave LB medium to prepare for cell culture.
  • Also, Ag+ incorporated clay is going to be filtered with water and ethanol to isolate clay from water/ethanol solution.

Procedure

  • 50mL LB media was made using the following way:
  0.50g Bacto-Trypsin
  0.25g Bacto-Yeast Extract
  0.50g Sodium Chloride
  50mL of distilled water
  Note: LB media obtained from Genova® with 2:1:2 proportion was used instead. 1.25g of LB powder was weighted out.
  • Each of the 50mL LB media was placed in two 125mL glass bottles and four 250mL Erlenmeyer flask.
  • 500mL of Agar was made using the following in a 1000mL Erlenmeyer flask:
  12.5g of LB media powder
  500mL of distilled water
  7.5g of Trypsin Soy Agar
  • The Erlenmeyer flask obtaining LB media or Agar were covered with aluminum foil, the bottles containing LB were capped loosely. All media were autoclaved under liquid cycle.
  • The clay+AgNO3 mixture was taken off hot plate.
  • A filter funnel, attached to 500mL Erlenmeyer flask then attached to a pump, is covered with a paper filter. Surface of paper filter was wetted and attached onto filter funnel through adding water.
  • The clay+AgNO3 mixture was then transfered into the filter funnel with suction applied.
  • Aluminum foil was used to cover the funnel to avoid silver to interact with light.

Results

  • The clay+AgNO3 mixture when taken off the hot plate appeared black. However, during filtration, the filtered liquid solution remained clear in color.



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