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 Experimental Biological Chemistry II
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Purpose
- Grind Ag+ Clay into powder for X-ray spectroscopic analysis.
- Grow DH5α-T1 bacteria cells and make two glycerol stocks of it.
Procedure
- Procedure for grinding of Ag+ Clay into powder form is recorded in Melissa's Notebook.
- Growing of DH5α-T1 cells
- One shot of DH5α-T1 cells was taken out from -87°C fridge.
- Cells were thawed in room temperature
- 3mL of autoclaved LB media was pipetted into a test tube
- Inoculation loop was used to obtain a small portion of cells. The cells were then dipped into the 3mL LB media.
- The cell culture was then placed in orbital shaker for 2 hours, at 37°C, with shaking at 230rpm.
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Experimental Biological Chemistry II
