User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/02/05: Difference between revisions
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==Procedure== | ==Procedure== | ||
* Procedure for grinding of Ag+ Clay into powder form is recorded in [[Melissa's Notebook]]. | * Procedure for grinding of Ag+ Clay into powder form is recorded in [[User:Melissa Novy/Notebook/CHEM-572/2013/02/05|Melissa's Notebook]]. | ||
* Growing of DH5α-T1 cells | * Growing of DH5α-T1 cells | ||
** One shot of DH5α-T1 cells was taken out from -87°C fridge. | ** One shot of DH5α-T1 cells was taken out from -87°C fridge. | ||
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** Inoculation loop was used to obtain a small portion of cells. The cells were then dipped into the 3mL LB media. | ** Inoculation loop was used to obtain a small portion of cells. The cells were then dipped into the 3mL LB media. | ||
** The cell culture was then placed in orbital shaker for 2 hours, at 37°C, with shaking at 230rpm. | ** The cell culture was then placed in orbital shaker for 2 hours, at 37°C, with shaking at 230rpm. | ||
** After two hours, the culture appeared clear, indicating no cell growth, an alternative approach were taken. | |||
* A different cell line was used for cell amplification, and the procedure for DH5α-T1 cell amplification was altered then repeated. | |||
** DH10B cells were taken from -87°C fridge. | |||
** The cell were let thaw on ice. | |||
** In two clean 15mL falcon tubes, 3mL autoclaved LB media were added. | |||
** In one falcon tube, 10uL DH10B cells were added. | |||
** In another falcon tube, 10uL DH5α-T1 cells were added. | |||
** The two falcon tubes were placed in orbital shaker for 2 hours, at 37°C, with shaking at 230rpm. | |||
** After 2 hours of incubation, the both cultures appeared clear. As a result, both cultures were placed in incubator horizontally to maximize shaking and left in shaker under same conditions for over night. | |||
Revision as of 17:21, 5 February 2013
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Purpose
Procedure
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