User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/02/05: Difference between revisions
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** In another falcon tube, 10uL DH5α-T1 cells were added. | ** In another falcon tube, 10uL DH5α-T1 cells were added. | ||
** The two falcon tubes were placed in orbital shaker for 2 hours, at 37°C, with shaking at 230rpm. | ** The two falcon tubes were placed in orbital shaker for 2 hours, at 37°C, with shaking at 230rpm. | ||
** After 2 hours of incubation, the both cultures appeared clear. As a result, both cultures were placed in incubator horizontally to maximize shaking and left in shaker under same conditions for over night. | |||
Revision as of 17:21, 5 February 2013
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Purpose
Procedure
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