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 Experimental Biological Chemistry II
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Purpose
- Make starter culture of DH5α-T1 cells in order to start pilot study.
Procedure
- DH5α-T1 plate was taken out this morning and placed in 20°C refrigerator
- In a sterile environment, 3mL of autoclaved LB was transferred into a 15mL falcon tube.
- With an autoclaved wooden stick, a cell colony from cell plate was obtained and dipped into the 3mL LB.
- The cell containing LB media was then placed into an orbital shaker at 230rpm for 16 hours.
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Experimental Biological Chemistry II
