User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/02/14: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Experimental Biological Chemistry</span>
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==Entry title==
==Purpose==
* Insert content here...
* To harvest cells grown on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/02/13|2013/02/13]] and store pellet in -80°C freezer for future use.
 
==Procedure==
* Cells were taken off the orbital shaker and were centrifuged at 4700rpm, at 4°C, for 30 minutes.
* The supernatent was poured out, leaving the pellet alone.
* The pellet was placed in -80°C freezer.




Latest revision as of 22:28, 26 September 2017

Experimental Biological Chemistry Main project page
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Purpose

  • To harvest cells grown on 2013/02/13 and store pellet in -80°C freezer for future use.

Procedure

  • Cells were taken off the orbital shaker and were centrifuged at 4700rpm, at 4°C, for 30 minutes.
  • The supernatent was poured out, leaving the pellet alone.
  • The pellet was placed in -80°C freezer.



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