User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/02/14

Purpose

• To harvest cells grown on 2013/02/13 and store pellet in -80°C freezer for future use.

Procedure

• Cells were taken off the orbital shaker and were centrifuged at 4700rpm, at 4°C, for 30 minutes.
• The supernatent was poured out, leaving the pellet alone.
• The pellet was placed in -80°C freezer.