User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/02/19: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Experimental Biological Chemistry I</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Entry title==
==Purpose==
* Insert content here...
* To make starter cultures of DH5α-T1 cells and autoclaved LB media for future use.
 
==Procedure==
* One colony of DH5α-T1 cell was inoculated with a sterile wooden stick and dipped into 3mL of sterile LB media inside a 15mL falcon tube.
* This process was repeated three more times to obtain a total of four cell culture-containing falcon tubes.
* The culture were placed in orbital shaker at 230rpm, at 37°C, for 15 hours.
* 12.5g of LB media was weighted out and placed in a 250mL Erlenmeyer flask.
* 50mL of distilled water was weighted out and poured into the Erlenmeyer flask.
* This process was repeated five more times to obtain a total of six LB-containing flasks.
* The flasks were placed in autoclave under liquid cycle.




Revision as of 13:18, 19 February 2013

Experimental Biological Chemistry I <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Purpose

  • To make starter cultures of DH5α-T1 cells and autoclaved LB media for future use.

Procedure

  • One colony of DH5α-T1 cell was inoculated with a sterile wooden stick and dipped into 3mL of sterile LB media inside a 15mL falcon tube.
  • This process was repeated three more times to obtain a total of four cell culture-containing falcon tubes.
  • The culture were placed in orbital shaker at 230rpm, at 37°C, for 15 hours.
  • 12.5g of LB media was weighted out and placed in a 250mL Erlenmeyer flask.
  • 50mL of distilled water was weighted out and poured into the Erlenmeyer flask.
  • This process was repeated five more times to obtain a total of six LB-containing flasks.
  • The flasks were placed in autoclave under liquid cycle.



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