User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/02/27: Difference between revisions
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==Procedure== | ==Procedure== | ||
* | * DH5α-T1 cell pellets were taken out from -80°C freezer and left in room temperature to defrost. | ||
* Under a sterile environment, 3mL of autoclaved LB medium were added onto pellet. The solution was pipetted up and down gently to mix. | * Under a sterile environment, 3mL of autoclaved LB medium were added onto pellet. The solution was pipetted up and down gently to mix. | ||
* In five bottles of 125mL Erlenmeyer flasks containing 50mL LB medium, the following amount of LB were taken out of the medium from each flask: | * In five bottles of 125mL Erlenmeyer flasks containing 50mL LB medium, the following amount of LB were taken out of the medium from each flask: |
Revision as of 19:59, 27 February 2013
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Purpose
Procedure
4.76uL, 5.00uL, 50.0uL, 500uL, 5000uL
Results
Observation
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