User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/03/05: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Experimental Biological Chemistry</span> | ||
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | ||
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== | ==Purpose== | ||
* | * Clean up bacteria cells from last week. | ||
* Autoclave LB for future use. | |||
==Procedure== | |||
* Bacteria cells were taken from orbital shaker. In each flask containing cells, 4-5 drops of 1M Wescodyne was added. The flasks were gently swirled to mix. | |||
* Flasks were left sitting for 4-5 minutes. Liquid in flasks were then poured into Silver Nitrate waste bottle located in Beeghly 207 under fume hood. | |||
* Flasks were cleaned and dried. | |||
* 16 Erlenmeyer flasks, each containing 50mL autoclaved LB, were needed for future studies: | |||
1 flask for negative control (cells only) | |||
1 flask for negative control (none) | |||
3 flasks for 50mL AgNO<sub>3</sub> with cells | |||
3 flasks for 25mL AgNO<sub>3</sub> with cells | |||
3 flasks for 75mL AgNO<sub>3</sub> with cells | |||
1 flask for 50mL AgNO<sub>3</sub> alone | |||
1 flask for 25mL AgNO<sub>3</sub> alone | |||
1 flask for 75mL AgNO<sub>3</sub> alone | |||
1 flask for 10mL AgNO<sub>3</sub> alone | |||
1 flask for 100mL AgNO<sub>3</sub> alone | |||
* 12.5g of LB media were weighted out and placed in each 250mL Erlenmeyer flask, and mixed with 50mL sterile water. | |||
* All flasks were autoclaved under liquid cycle. | |||
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Revision as of 07:20, 11 March 2013
Experimental Biological Chemistry | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Purpose
Procedure
1 flask for negative control (cells only) 1 flask for negative control (none) 3 flasks for 50mL AgNO3 with cells 3 flasks for 25mL AgNO3 with cells 3 flasks for 75mL AgNO3 with cells 1 flask for 50mL AgNO3 alone 1 flask for 25mL AgNO3 alone 1 flask for 75mL AgNO3 alone 1 flask for 10mL AgNO3 alone 1 flask for 100mL AgNO3 alone
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