User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/04/03: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Experimental Biological Chemistry</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Experimental Biological Chemistry II</span> | ||
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | ||
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==Purpose== | ==Purpose== | ||
* Prepare films for cell growth study on Sunday. | * Prepare films for cell growth study on Sunday. | ||
* Prepare starter culture. | |||
==Procedure== | ==Procedure== | ||
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* From the dried films, four pieces of 0.5g films in circular shapes were cut out from each film. | * From the dried films, four pieces of 0.5g films in circular shapes were cut out from each film. | ||
* Films were placed in small plastic bag for storage. | * Films were placed in small plastic bag for storage. | ||
* Cells were harvested from shaker. The cells were placed in centrifuge to spin at 4°C, 4700rpm, for 30 minutes. | |||
* Supernatents were discarded for each falcon tube. | |||
* Pellets were placed in -80°C freezer until use. | |||
Revision as of 20:55, 7 April 2013
Experimental Biological Chemistry II | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Purpose
Procedure
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