User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/04/07

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Autocreate 2013/04/07 Entry for User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I)
Line 6: Line 6:
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-
==Entry title==
+
==Purpose==
-
* Insert content here...
+
* Run 15 hour cell growth experiment with silver incorporated clay/polymer film and clay/polymer film made on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/04/03|2013/04/03]]
 +
 
 +
==Procedure==
 +
* Starter culture was thawed in room temperature.
 +
* 3mL of sterile LB media was pipetted into falcon tube to resuspend pellet
 +
* 1mL of resuspended culture was pipetted into each 50mL sterilized LB.
 +
* Above two processes were repeated for six more times to make a total of 7 cell cultures in 7 flasks.
 +
* First flask including LB and cell serve as control.
 +
* One of each LMT+AG200D film was placed in three other flasks, respectively.
 +
* One of each AG-incorporated LMT+AG200D film was placed in the last three flasks, respectively.
 +
* All flasks were placed in incubator shaker for 15 hours, 230rpm, at 37°C.
 +
* Absorbance of 3mL cell culture was taken every three hours using UV-vis spectrometer at 600.0nm.
 +
* The absorbance of all cell culture was measured against water.
 +
* Results were shown in results section.
 +
 
 +
==Results==
 +
* Absorbance of LB is 0.7 at 600nm.
 +
* See table below for data point collected.
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''Hour'''
 +
| align="center" style="background:#f0f0f0;"|'''Control (LB+cell)'''
 +
| align="center" style="background:#f0f0f0;"|'''LB+Cell+clay/polymer film #1'''
 +
| align="center" style="background:#f0f0f0;"|'''LB+Cell+clay/polymer film #2'''
 +
| align="center" style="background:#f0f0f0;"|'''LB+Cell+clay/polymer film #3'''
 +
| align="center" style="background:#f0f0f0;"|'''LB+cell+silver incorporated clay/polymer film #1'''
 +
| align="center" style="background:#f0f0f0;"|'''LB+cell+silver incorporated clay/polymer film #2'''
 +
| align="center" style="background:#f0f0f0;"|'''LB+cell+silver incorporated clay/polymer film #3'''
 +
|-
 +
| 0||0.107||0.107||0.12||0.111||0.116||0.109||0.126
 +
|-
 +
| 3||0.249||0.143||0.157||0.152||0.133||0.235||0.295
 +
|-
 +
| 6||1.257||0.786||0.781||0.845||1.34||1.225||0.946
 +
|-
 +
| 9||1.927||1.854||1.987||1.874||1.972||1.906||2.011
 +
|-
 +
| 12||2.122||2.131||2.212||2.148||2.101||2.138||2.166
 +
|-
 +
| 15||2.203||2.314||2.26||2.243||2.178||2.157||2.211
 +
|}
 +
* Graph below shows the relationship between cell growth and time.

Revision as of 22:47, 7 April 2013

Project name Main project page
Previous entry      Next entry

Purpose

  • Run 15 hour cell growth experiment with silver incorporated clay/polymer film and clay/polymer film made on 2013/04/03

Procedure

  • Starter culture was thawed in room temperature.
  • 3mL of sterile LB media was pipetted into falcon tube to resuspend pellet
  • 1mL of resuspended culture was pipetted into each 50mL sterilized LB.
  • Above two processes were repeated for six more times to make a total of 7 cell cultures in 7 flasks.
  • First flask including LB and cell serve as control.
  • One of each LMT+AG200D film was placed in three other flasks, respectively.
  • One of each AG-incorporated LMT+AG200D film was placed in the last three flasks, respectively.
  • All flasks were placed in incubator shaker for 15 hours, 230rpm, at 37°C.
  • Absorbance of 3mL cell culture was taken every three hours using UV-vis spectrometer at 600.0nm.
  • The absorbance of all cell culture was measured against water.
  • Results were shown in results section.

Results

  • Absorbance of LB is 0.7 at 600nm.
  • See table below for data point collected.
Hour Control (LB+cell) LB+Cell+clay/polymer film #1 LB+Cell+clay/polymer film #2 LB+Cell+clay/polymer film #3 LB+cell+silver incorporated clay/polymer film #1 LB+cell+silver incorporated clay/polymer film #2 LB+cell+silver incorporated clay/polymer film #3
00.1070.1070.120.1110.1160.1090.126
30.2490.1430.1570.1520.1330.2350.295
61.2570.7860.7810.8451.341.2250.946
91.9271.8541.9871.8741.9721.9062.011
122.1222.1312.2122.1482.1012.1382.166
152.2032.3142.262.2432.1782.1572.211
  • Graph below shows the relationship between cell growth and time.



Personal tools