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Purpose
- Run 15 hour cell growth experiment with silver incorporated clay/polymer film and clay/polymer film made on 2013/04/03
Procedure
- Starter culture was thawed in room temperature.
- 3mL of sterile LB media was pipetted into falcon tube to resuspend pellet
- 1mL of resuspended culture was pipetted into each 50mL sterilized LB.
- Above two processes were repeated for six more times to make a total of 7 cell cultures in 7 flasks.
- First flask including LB and cell serve as control.
- One of each LMT+AG200D film was placed in three other flasks, respectively.
- One of each AG-incorporated LMT+AG200D film was placed in the last three flasks, respectively.
- All flasks were placed in incubator shaker for 15 hours, 230rpm, at 37°C.
- Absorbance of 3mL cell culture was taken every three hours using UV-vis spectrometer at 600.0nm.
- The absorbance of all cell culture was measured against water.
- Results were shown in results section.
Results
- Absorbance of LB is 0.7 at 600nm.
- See table below for data point collected.
| Hour
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Control (LB+cell)
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LB+Cell+clay/polymer film #1
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LB+Cell+clay/polymer film #2
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LB+Cell+clay/polymer film #3
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LB+cell+silver incorporated clay/polymer film #1
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LB+cell+silver incorporated clay/polymer film #2
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LB+cell+silver incorporated clay/polymer film #3
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| 0 |
0.107 |
0.107 |
0.12 |
0.111 |
0.116 |
0.109 |
0.126
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| 3 |
0.249 |
0.143 |
0.157 |
0.152 |
0.133 |
0.235 |
0.295
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| 6 |
1.257 |
0.786 |
0.781 |
0.845 |
1.34 |
1.225 |
0.946
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| 9 |
1.927 |
1.854 |
1.987 |
1.874 |
1.972 |
1.906 |
2.011
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| 12 |
2.122 |
2.131 |
2.212 |
2.148 |
2.101 |
2.138 |
2.166
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| 15 |
2.203 |
2.314 |
2.26 |
2.243 |
2.178 |
2.157 |
2.211
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- Graph below shows the relationship between cell growth and time.
[Image:15 hours experiment.png|600px]
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