User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/04/22: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Experimental Biological Chemistry II</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Entry title==
==Purpose==
* Insert content here...
* DAY 6 - Continue with running the 8-day study on cell growth in media containing films that has been soaking for 2 to 8 days.


==Procedure==
* The flasks were taken out from orbital shaker.
* 3mL of cell culture were taken from each flasks in a 4mL glass cuvette.
* The absorbance for the 3mL cell culture was taken with wavelength 600.0nm.
* Absorbances were recorded.
* The above three procedures were repeated every three hours for a time span of 15 hours.


==Results==
* A 15-Hour Study on Cell Growth on 6th Day of the 8-day Cell Growth Experiment graph was plotted. The graph is plotted with absorbance at 600nm against time in 2-hour increments.
* In flasks containing the control film and without film, absorbance increases throughout time and eventually plateaus. However, in flask containing silver-incorporated film, the absorbance remained low until the 9th hour, then increases exponentially.
[[Image:15-hour study on cell growth.png|600px]]
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Revision as of 10:23, 30 April 2013

Experimental Biological Chemistry II <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Purpose

  • DAY 6 - Continue with running the 8-day study on cell growth in media containing films that has been soaking for 2 to 8 days.

Procedure

  • The flasks were taken out from orbital shaker.
  • 3mL of cell culture were taken from each flasks in a 4mL glass cuvette.
  • The absorbance for the 3mL cell culture was taken with wavelength 600.0nm.
  • Absorbances were recorded.
  • The above three procedures were repeated every three hours for a time span of 15 hours.

Results

  • A 15-Hour Study on Cell Growth on 6th Day of the 8-day Cell Growth Experiment graph was plotted. The graph is plotted with absorbance at 600nm against time in 2-hour increments.
  • In flasks containing the control film and without film, absorbance increases throughout time and eventually plateaus. However, in flask containing silver-incorporated film, the absorbance remained low until the 9th hour, then increases exponentially.


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