User:Khyra A. Neal/Notebook/Chem 571/2014/09/16: Difference between revisions

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[[Image:GlycineCalibration.jpg]]


[[Image:GlycineConductivity.jpg]]
[[Image:NaClCalibration.jpg]]
[[Image:NaClConductivity.jpg]]


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Revision as of 20:32, 17 September 2014

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September 16, 2014

Tasklist

1. Prepare 500 mL 50mM NaCl

  • (50 X 10-3M NaCl) * (58.44 g/mol) * (1 L/1000 mL) * 500 mL = 1.461 g NaCl for 500 mL

2. Prepare 1 L 50 mM Glycine Buffer at pH 3.5

  • 50 X 10-3M Glycine) * (75.07 g/mol) = 3.754 g in 1 L
  • Add 4 mL 1 M HCl to lower pH to 3.5

3. Prepare 25 mL 1 g/L Lysozyme

  • 0 .025 g Lysozyme in 25 mL

Dialysis

1. Dialyze 4 mL of Lysozyme stock solution (solution that was prepared in previous steps) in 3 in dialysis tube

  • 3500 g/mol MWCO tubing in approximately 100 mL of each solution
    • NaCl
    • Glycine
    • Pure Soak Water (HPLC Water)
  • 25k g/mol MWCO tubing in Glycine soak

2. Dialyze 4 mL of Lysozyme colloid solution in 3500 g/mol MWCO tubing for glycine soak.

pH and Conductivity

  • Prepare 2x, 5x, 10x, 20x, 50x, 100x, 200x, and 500x dilutions of 50 mM Glycine and NaCl stock solutions.
  • The pH and conductivity measurements are summarized in the tables and graphs below.

NaCl (mM) pH Conductivity (μS/cm)
0.1 * 6.768 50.8
0.25 6.331 75.4
0.5 6.507 68.0
1 6.188 290.2
2.5 5.989 304
5 6.189 623
10 5.963 1178
25 6.064 3030


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