User:Khyra A. Neal/Notebook/Chem 571/2014/09/24

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September 25,2014

High Pressure Liquid Chromatography (HPLC)

Running HPLC with Gel Permeation Column (GPC)

Tasklist

1. Prepare 0.5 g/L and 0.1 g/L Lysozyme from 1 g/L Lysozyme Stock

  • (1 g/L)v1= (0.5 g/L)(10 mL)
    • Volume of 1 g/L Lysozyme needed: 5 mL need
    • Dilute to 10 mL in volumetric flask
  • (1 g/L)v1= (0.1 g/L)(10 mL)
    • Volume of 1 g/L Lysozyme needed: 1 mL
    • Dilute to 10 mL in volumetric flask

2. Prepare 0.5 M acetic acid and 0.3 M Na2SO4

  • 0.5mol HAc/L)(0.5 L)(60.05 g/mol)(1mL/1.05g)=14.3mL of acetic acid needed
  • (0.3mol NaSO4/L)(0.5L)(142.04g/mol)=21.3060 g of NaSO4 need to be added to 500 mL volumetric flask under fume hood
    • Add 14.3 mL of acetic acid to 500 mL volumetric flask (ADD SMALL AMOUNT OF HPLC WATER FIRST)
    • Dilute to 500 mL with HPLC water.



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