User:Khyra A. Neal/Notebook/Chem 571/2014/09/30: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
==Tasklist== | ==September 30, 2014== | ||
===Tasklist=== | |||
1. Calibration Curve for CaCl<sub>2</sub> | 1. Calibration Curve for CaCl<sub>2</sub> | ||
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* Prepare 25 mL of each using serial dilutions: 5 mM, 500 μM, 50 μM, and 5 μM CaCl<sub>2</sub> from 50 mM stock CaCl<sub>2</sub> | * Prepare 25 mL of each using serial dilutions: 5 mM, 500 μM, 50 μM, and 5 μM CaCl<sub>2</sub> from 50 mM stock CaCl<sub>2</sub> | ||
** Dilute all solutions to 25 mL with HPLC water. | ** Dilute all solutions to 25 mL with HPLC water. | ||
*Measure | *Measure Ca<sup>2+</sup> using ISE | ||
{| {{table}} | {| {{table}} | ||
| align="center" style="background:#f0f0f0;"|'''CaCl<sub>2</sub> concentration''' | | align="center" style="background:#f0f0f0;"|'''CaCl<sub>2</sub> concentration''' | ||
| align="center" style="background:#f0f0f0;"|''' | | align="center" style="background:#f0f0f0;"|'''Potential (mV)''' | ||
|- | |- | ||
| 5μM || -44.6 | | 5μM || -44.6 | ||
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{| {{table}} | {| {{table}} | ||
| align="center" style="background:#f0f0f0;"|'''Substance''' | | align="center" style="background:#f0f0f0;"|'''Substance''' | ||
| align="center" style="background:#f0f0f0;"|''' | | align="center" style="background:#f0f0f0;"|'''Potential (mV)''' | ||
|- | |- | ||
| Tap water || 51.3 | | Tap water || 51.3 | ||
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|} | |} | ||
* Measure 50 mM and 50 mM of other salts to check for interferences | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''CaCl<sub>2</sub> concentration''' | |||
| align="center" style="background:#f0f0f0;"|'''Cu(II)Cl<sub>2</sub> concentration''' | |||
| align="center" style="background:#f0f0f0;"|'''KCl concentration''' | |||
| align="center" style="background:#f0f0f0;"|'''Potential (mV)''' | |||
|- | |||
| 50mM || 0||50mM||86.4 | |||
|- | |||
| 50mM || 50mM||0||80.5 | |||
|} | |||
2. Calibration Curve for NaCl | 2. Calibration Curve for NaCl | ||
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{| {{table}} | {| {{table}} | ||
| align="center" style="background:#f0f0f0;"|'''NaCl concentration''' | | align="center" style="background:#f0f0f0;"|'''NaCl concentration''' | ||
| align="center" style="background:#f0f0f0;"|''' | | align="center" style="background:#f0f0f0;"|'''Potential (mV)''' | ||
|- | |- | ||
| 5μM || 146.5 | | 5μM || 146.5 | ||
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{| {{table}} | {| {{table}} | ||
| align="center" style="background:#f0f0f0;"|'''Substance''' | | align="center" style="background:#f0f0f0;"|'''Substance''' | ||
| align="center" style="background:#f0f0f0;"|''' | | align="center" style="background:#f0f0f0;"|'''Potential (mV)''' | ||
|- | |- | ||
| Tap water || 65.5 | | Tap water || 65.5 | ||
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3. Try measuring 50 mM and 50 mM of other salts to check for interferences | 3. Try measuring 50 mM and 50 mM of other salts to check for interferences | ||
{| {{table}} | {| {{table}} | ||
| align="center" style="background:#f0f0f0;"|'''NaCl concentration''' | | align="center" style="background:#f0f0f0;"|'''NaCl concentration''' | ||
| align="center" style="background:#f0f0f0;"|''' | | align="center" style="background:#f0f0f0;"|'''NaSO<sub>4</sub>''' | ||
| align="center" style="background:#f0f0f0;"|''' | | align="center" style="background:#f0f0f0;"|'''Na<sub>2</sub>HPO<sub>4</sub><sup>2-</sup>''' | ||
| align="center" style="background:#f0f0f0;"|'''Potential (mV)''' | |||
|- | |||
| 50mM || 50 mM||0||-27.0 | |||
|- | |- | ||
| | | 50mM || 0||50mM||-30.8 | ||
|- | |- | ||
|} | |} |
Latest revision as of 00:21, 27 September 2017
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September 30, 2014Tasklist1. Calibration Curve for CaCl2
Dialysis
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