User:Khyra A. Neal/Notebook/Chem 571/2014/09/30: Difference between revisions

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*Measure Ca<sup>2+</sup> using ISE  
*Measure Ca<sup>2+</sup> using ISE  


{| {{table}}
| align="center" style="background:#f0f0f0;"|
| align="center" style="background:#f0f0f0;"|'''Conductivity'''
| align="center" style="background:#f0f0f0;"|
|-
| Concentration||NaCl||CaCl<sub>2</sub>
|-
| 5 μM||146.5 mV||44.6 mV
|-
| 50 μM||129.6 mV||8.2 mV
|-
| 500 μM||81.3 mV||39.5 mV
|-
| 5 mM||27.5 mV||63.5 mV
|-
| 50 mM||23.9 mV||86.6 mV
|}


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Revision as of 12:12, 30 September 2014

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Tasklist

1. Calibration Curve for CaCl2

  • Prepare 25 mL 50 mM CaCl2 using HPLC water
    • 0.13875 g CaCl2 in 25 mL HPLC water
  • Prepare 25 mL of each using serial dilutions: 5 mM, 500 μM, 50 μM, and 5 μM CaCl2 from 50 mM stock CaCl2
    • Dilute all solutions to 25 mL with HPLC water.
  • Measure Cl- using ISE


2. Calibration Curve for NaCl

  • Prepare 25 mL of 50 mM NaCl using HPLC water
    • 0.07305 g NaCl in 25 mL HPLC water
  • Prepare 25 mL of each using serial dilutions: 5 mM, 500 μM, 50 μM, and 5 μM NaCl from 50 mM NaCl stock solution
    • Dilute all solutions to 25 mL with HPLC water
  • Measure Ca2+ using ISE
Conductivity
Concentration NaCl CaCl2
5 μM 146.5 mV 44.6 mV
50 μM 129.6 mV 8.2 mV
500 μM 81.3 mV 39.5 mV
5 mM 27.5 mV 63.5 mV
50 mM 23.9 mV 86.6 mV


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