Tasklist
1. Calibration Curve for CaCl2
- Prepare 25 mL 50 mM CaCl2 using HPLC water
- 0.13875 g CaCl2 in 25 mL HPLC water
- Prepare 25 mL of each using serial dilutions: 5 mM, 500 μM, 50 μM, and 5 μM CaCl2 from 50 mM stock CaCl2
- Dilute all solutions to 25 mL with HPLC water.
- Measure Cl- using ISE
| CaCl2 concentration
|
mV measurement (mV)
|
| 5μM |
-44.6
|
| 50μM |
8.2
|
| 500μM |
39.5
|
| 5mM |
63.5
|
| 50mM |
86.6
|
- Measure HPLC water, DI water, tap water, and your lysozyme stock solution.
| Substance
|
mV measurement (mV)
|
| Tap water |
51.3
|
| HPLC water |
-23.0
|
| DI water |
-51.5
|
| Lysozyme 0.5g/L |
-19.1
|
2. Calibration Curve for NaCl
- Prepare 25 mL of 50 mM NaCl using HPLC water
- 0.07305 g NaCl in 25 mL HPLC water
- Prepare 25 mL of each using serial dilutions: 5 mM, 500 μM, 50 μM, and 5 μM NaCl from 50 mM NaCl stock solution
- Dilute all solutions to 25 mL with HPLC water
- Measure Cl- using ISE
| NaCl concentration
|
mV measurement (mV)
|
| 5μM |
146.5
|
| 50μM |
129.6
|
| 500μM |
81.3
|
| 5mM |
27.5
|
| 50mM |
-23.9
|
- Measure HPLC water, DI water, tap water, and your lysozyme stock solution.
| Substance
|
mV measurement (mV)
|
| Tap water |
65.5
|
| HPLC water |
163.5
|
| DI water |
158.8
|
| Lysozyme 0.5g/L |
89.4
|
3. Try measuring 50 mM and 50 mM of other salts to check for interferences
- Prepare 25 mL of each salt mixture
- Cupric Chloride = 0.16806 g
- NaCl= 0.073 g
- Dilute to 25 mL with HPLC water
|
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