User:Khyra A. Neal/Notebook/Chem 571/2014/10/15: Difference between revisions
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The original protocol for electrophoresis was written by [http://openwetware.org/wiki/User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2013/09/25 Dr. Hartings] | The original protocol for electrophoresis was written by [http://openwetware.org/wiki/User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2013/09/25 Dr. Hartings] | ||
2. Determination of Dialyzed KI Samples extracted on [http://openwetware.org/wiki/User:Khyra_A._Neal/Notebook/Chem_571/2014/10/08 October 14, 2014] | 2. Determination of Dialyzed KI Samples extracted on [http://openwetware.org/wiki/User:Khyra_A._Neal/Notebook/Chem_571/2014/10/08 October 14, 2014] |
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October 15, 2014Tasklist1. Electrophoresis
The original protocol for electrophoresis was written by Dr. Hartings
2. Determination of Dialyzed KI Samples extracted on October 14, 2014
Standardization: AgNO3 + NH4SCN → AgSCN + NH4NO3 KI + AgNO3(excess) → AgI (s) + AgNO3 +KNO3 Initial AgNO3: 0.075 M AgNO3 x 0.002 L AgNO3 = 0.00015 mol AgNO3 AgNO3 (neutralized): 0.00968 M NH4SCN x 0.0104 L NH4SCN x (1 mol AgNO3 / 1 mol NH4SCN ) = 0.000100672 mol AgNO3 Moles of KI: 0.00015 mol AgNO3 initial - 0.000100672 mol AgNO3 neutralized = 0.0000493 mol KI [KI]= 0.0000493 mol KI / 0.001 L KI = 49.3 mM KI NOTE The final concentration of NH4SCN was determined by titration standardization on October 8, 2014
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