User:Khyra A. Neal/Notebook/Chem 571/2014/10/21: Difference between revisions
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== | ==October 21, 2014== | ||
* | ===Tasklist=== | ||
1. Preparation of Dialysis for 30:1 Colloid vs CaCl2 | |||
2. Prepare ISE for Iodide | |||
3. Determine Molecular weight of Electrophoresis gel from [http://openwetware.org/wiki/User:Khyra_A._Neal/Notebook/Chem_571/2014/10/15 October 15, 2014] | |||
4. Prepare 10 mL 3 M NaCl | |||
* Carefully remove yellow plastic covering on Ag/AgCl electrode (as instructed) | |||
* Place electrode in 15 mL falcon tube with enough NaCl solution to match volume in electrode | |||
* cover and store upright until needed | |||
5. Prepare 50 mL 4 M KNO3 | |||
* Add KNO3 to outer double junction cell, filling narrow tube + ~ 0.5 inches further | |||
Place electrode in small vial with enough KNO3 solution to cover the vycor tip | |||
* Prepare 50 mL 0.01 M KI + 0.1 M KNO3 | |||
* Cut a short piece (~ 3 in) of silver wire | |||
* Place in KI/KNO3 solution | |||
* Electrolyze the solution using the power supply | |||
* The standard reduction potential for Ag/AgI is -0.15 V | |||
* Oxidation occurs at the anode, which is connected to the red lead | |||
* Electrolyze at low power (low current) until the wire appears coated | |||
6. Test your electrodes with a range of KI solutions | |||
* The Ag/AgCl electrode slides inside of the KNO3 bridge solution (this is a double junction electrode) | |||
* The Ag/AgI wire can be connected directly to a multimeter using alligator clips | |||
* You will continue to connect your Ag wire to the red lead on the multimeter | |||
Revision as of 09:26, 21 October 2014
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October 21, 2014Tasklist1. Preparation of Dialysis for 30:1 Colloid vs CaCl2 2. Prepare ISE for Iodide 3. Determine Molecular weight of Electrophoresis gel from October 15, 2014 4. Prepare 10 mL 3 M NaCl
5. Prepare 50 mL 4 M KNO3
Place electrode in small vial with enough KNO3 solution to cover the vycor tip
6. Test your electrodes with a range of KI solutions
|