User:Khyra A. Neal/Notebook/Chem 571/2014/10/22: Difference between revisions
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===Tasklist=== | ===Tasklist=== | ||
* Extract Dialyzed 30:1 Colloid Solutions against CaCl<sub>2</sub> that were dialyzed on [http://openwetware.org/wiki/User:Khyra_A._Neal/Notebook/Chem_571/2014/10/21 October 21,2014] | |||
Bradford Analysis of Extracted Colloid Solutions | |||
* 100 μL sample | * 100 μL sample | ||
* 200 μL Bradford Reagent diluted (1:3) | * 200 μL Bradford Reagent diluted (1:3) | ||
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* Run UV-Vis between 400 nm and 800 nm | * Run UV-Vis between 400 nm and 800 nm | ||
3. UV-Vis Fluorescence | |||
There are no peaks at 280 nm for the dialyzed colloid solutions because phenylalanine, tryptophan, and tyrosine are reducing agents to gold. There is no free protein remaining in solution after dialysis. | |||
The diagrams below confirm the above statement. | |||
*Insert Diagram here* | |||
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Revision as of 11:25, 22 October 2014
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Ocotober 22, 2014Tasklist
Bradford Analysis of Extracted Colloid Solutions
3. UV-Vis Fluorescence There are no peaks at 280 nm for the dialyzed colloid solutions because phenylalanine, tryptophan, and tyrosine are reducing agents to gold. There is no free protein remaining in solution after dialysis. The diagrams below confirm the above statement.
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