User:Khyra A. Neal/Notebook/Experimental Biological Chemistry II Notebook/2015/01/27: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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==Stock Solution Preparation== | ==Stock Solution Preparation== | ||
1. Prepare 100 mL 2.5 mM HAuCl<sub>4</sub> | 1. Prepare 100 mL 2.5 mM HAuCl<sub>4</sub> at pH 3.6 | ||
* 2.5 mM HAuCl<sub>4</sub> * 14307 g HAuCl<sub>4</sub> / mol * 100 mL = '''0.085 g HAuCl<sub>4</sub>''' | * 2.5 mM HAuCl<sub>4</sub> * 14307 g HAuCl<sub>4</sub> / mol * 100 mL = '''0.085 g HAuCl<sub>4</sub>''' | ||
** 0.08533 g was the final amount weighed | ** 0.08533 g was the final amount weighed giving a final concentration of '''251 μM HAuCl<sub>4</sub>''' | ||
* Add 0.08533 g HAuCl<sub>4</sub> to a volumetric flask and dilute to 100 mL with deionized H<sub>2</sub>O | * Add 0.08533 g HAuCl<sub>4</sub> to a volumetric flask and dilute to 100 mL with deionized H<sub>2</sub>O | ||
** Monika prepared a total of 100 mLs HAuCl<sub>4</sub> for both groups. | ** Monika prepared a total of 100 mLs HAuCl<sub>4</sub> for both groups. | ||
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2. Prepare 50 mL 20 μM Lysozyme | 2. Prepare 50 mL 20 μM Lysozyme | ||
* 20 μM Lys * 340 g Lys /mol * 50 mL = '''0.014 g Lysozyme''' | * 20 μM Lys * 340 g Lys /mol * 50 mL = '''0.014 g Lysozyme''' | ||
** 0.01417 g was the final amount weighed giving a final concentration of '''19.81 μM Lysozyme | |||
* Add 0.014 g Lys to a volumetric flask and dilute to 50 mL with deionized H<sub>2</sub>O | * Add 0.014 g Lys to a volumetric flask and dilute to 50 mL with deionized H<sub>2</sub>O | ||
== Gold Colloid and Fiber Preparation== | |||
1. 30:1 Au:Lys Colloid | |||
* To prepare 5 mL add 0.5 mL 251 μM HAuCl<sub>4</sub> | |||
* Desired Lysozyme concentration= 250 μM HAuCl<sub>4</sub> / 30 = 8.3 μM Lys | |||
** M<sub>1</sub>V<sub>1</sub>=M<sub>2</sub>V<sub>2</sub> | |||
** 19.81 μM * V<sub>1</sub> = 8.3 μM * 5 mL = 2.095 mL Lys | |||
* Dilute with 2.405 mL deionized H<sub>2</sub>O | |||
** Final volume = 5 mL | |||
* Heat at 80°C for 4 hours | |||
2. 45:1 Au:Lys Fiber Formation | |||
* To prepare 5 mL add 0.5 mL 251 μM HAuCl<sub>4</sub> | |||
* Desired Lysozyme concentration= 251 μM HAuCl<sub>4</sub> / 45 = 5.56 μM Lys | |||
** M<sub>1</sub>V<sub>1</sub>=M<sub>2</sub>V<sub>2</sub> | |||
** 19.81 μM * V<sub>1</sub> = 5.56 μM * 5 mL = 1.40 mL Lys | |||
* Dilute with 3.10 mL deionized H<sub>2</sub>O (final volume = 5 mL) | |||
* Heat at 80°C for 4 hours | |||
There were a total of 10 30:1 Au:Lys colloid and 10 45:1 Au: Lys fiber test tubes prepared. | |||
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__NOTOC__ | __NOTOC__ |
Latest revision as of 00:41, 27 September 2017
Project name | Main project page Next entry |
January 27,2015Stock Solution Preparation1. Prepare 100 mL 2.5 mM HAuCl4 at pH 3.6
2. Prepare 50 mL 20 μM Lysozyme
Gold Colloid and Fiber Preparation1. 30:1 Au:Lys Colloid
2. 45:1 Au:Lys Fiber Formation
There were a total of 10 30:1 Au:Lys colloid and 10 45:1 Au: Lys fiber test tubes prepared. |