User:Khyra A. Neal/Notebook/Experimental Biological Chemistry II Notebook/2015/01/27

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January 27,2015 Stock Solution Preparation 1. Prepare 100 mL 2.5 mM HAuCl4 at pH 3.6 2.5 mM HAuCl4 * 14307 g HAuCl4 / mol * 100 mL = 0.085 g HAuCl4 0.08533 g was the final amount weighed giving a final concentration of 251 μM HAuCl4 Add 0.08533 g HAuCl4 to a volumetric flask and dilute to 100 mL with deionized H2O Monika prepared a total of 100 mLs HAuCl4 for both groups. 2. Prepare 50 mL 20 μM Lysozyme 20 μM Lys * 340 g Lys /mol * 50 mL = 0.014 g Lysozyme 0.01417 g was the final amount weighed giving a final concentration of 19.81 μM Lysozyme Add 0.014 g Lys to a volumetric flask and dilute to 50 mL with deionized H2O Gold Colloid and Fiber Preparation 1. 30:1 Au:Lys Colloid To prepare 5 mL add 0.5 mL 251 μM HAuCl4 Desired Lysozyme concentration= 250 μM HAuCl4 / 30 = 8.3 μM Lys M1V1=M2V2 19.81 μM * V1 = 8.3 μM * 5 mL = 2.095 mL Lys Dilute with 2.405 mL deionized H2O Final volume = 5 mL Heat at 80°C for 4 hours 2. 45:1 Au:Lys Fiber Formation To prepare 5 mL add 0.5 mL 251 μM HAuCl4 Desired Lysozyme concentration= 251 μM HAuCl4 / 45 = 5.56 μM Lys M1V1=M2V2 19.81 μM * V1 = 5.56 μM * 5 mL = 1.40 mL Lys Dilute with 3.10 mL deionized H2O (final volume = 5 mL) Heat at 80°C for 4 hours There were a total of 10 30:1 Au:Lys colloid and 10 45:1 Au: Lys fiber test tubes prepared.