User:Khyra A. Neal/Notebook/Experimental Biological Chemistry II Notebook/2015/02/24: Difference between revisions

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==Objective==
==Objective==
# Start new Reaction
# Start new Reaction
#Prepare More 30:1 Au:Lys colloid
#Prepare More 30:1 Au:Lys colloid and 45:1 Au:Lys nanofibers
# Dry nanofibers


==Description==
#Begin Reaction
## Oxidation of Benzhydrol
##* 0.614 g Benzhydrol transferred to 10 mL volumetric flask and dissolved in ethanol
##* 0.333 mL 30% H<sub>2</sub>O<sub>2</sub> was added to volumetric flask
##* The solution was diluted with ethanol to 10 mL
## 3 mL of the solution was added to a 50 mL pear bottom flask and attached to a reflux condenser on a schlenk line
##* Nitrogen gas was added to the flask to removed any oxygen remaining in the solution
##* 23.03 mL 30:1 Au:Lys colloid was added to the reaction mixture through septum
##* The reaction mixture was constantly stirred in an oil bath at 75°C for 8 hours.
## Water was added to the reaction mixture and continuously stirred at room temperature for the remainder of the day
# Prepare Colloid and Nanofibers
## 30:1 Au:Lys Colloid
##* 2.095 mL Lysozyme, 0.5 mL HAuCl<sub>4</sub> and 2.405 mL H<sub>2</sub>O was added to 40 test tubes
## 45:1 Au:Lys Nanofibers
##* 1.40 mL Lysozyme, 0.5 mL HAuCl<sub>4</sub> and 3.10 mL H<sub>2</sub>O was added to 40 test tubes
## The test tubes were heated for 4 hours at 80°C
#
==Notes==
There wasn't enough nitrogen in the tank to provide a continuous flow of nitrogen through the shlenk line.


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February 24,2015

Objective

  1. Start new Reaction
  2. Prepare More 30:1 Au:Lys colloid and 45:1 Au:Lys nanofibers
  3. Dry nanofibers

Description

  1. Begin Reaction
    1. Oxidation of Benzhydrol
      • 0.614 g Benzhydrol transferred to 10 mL volumetric flask and dissolved in ethanol
      • 0.333 mL 30% H2O2 was added to volumetric flask
      • The solution was diluted with ethanol to 10 mL
    2. 3 mL of the solution was added to a 50 mL pear bottom flask and attached to a reflux condenser on a schlenk line
      • Nitrogen gas was added to the flask to removed any oxygen remaining in the solution
      • 23.03 mL 30:1 Au:Lys colloid was added to the reaction mixture through septum
      • The reaction mixture was constantly stirred in an oil bath at 75°C for 8 hours.
    3. Water was added to the reaction mixture and continuously stirred at room temperature for the remainder of the day
  2. Prepare Colloid and Nanofibers
    1. 30:1 Au:Lys Colloid
      • 2.095 mL Lysozyme, 0.5 mL HAuCl4 and 2.405 mL H2O was added to 40 test tubes
    2. 45:1 Au:Lys Nanofibers
      • 1.40 mL Lysozyme, 0.5 mL HAuCl4 and 3.10 mL H2O was added to 40 test tubes
    3. The test tubes were heated for 4 hours at 80°C



Notes

There wasn't enough nitrogen in the tank to provide a continuous flow of nitrogen through the shlenk line.


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