User:Klare Lazor/Notebook/Chem-496-001/2011/08/31: Difference between revisions
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==Description== | ==Description== | ||
Protocol: | |||
Materials needed included Chloroauric acid HAuCl4, bovine serum albumen (BSA), and zein protein. BSA(0.0015-0.015mM) and HAuCl4(0.25-10mM) were used to create a 10 mL aqueous mixtures. Each mixture was transferred to a screw-capped glass bottle. The mixtures were then kept in a water thermostat bath at 40, 60, or 80±1˚C for 6 hours. The solutions color changed from colorless to pink-purple and final purple. The samples were then removed from the bath and left to cool overnight. They were purified with distilled water two times in order to remove any excess BSA, using a centrifuge at 10000-14000rpm for 5 minutes. | |||
In order to determine the absorbance caused by surface plasmon resonance, UV-visible spectra were taken of each sample using an UV Spectrophotometer with a wavelength range of 200 to 900 nm. Some time dependent scans were also taken to understand the growth kinetics of Au nanoparticles. Finally, samples were placed in different water temperature baths (20 to 70˚C) to observe how temperature affects the synthesis of nanoparticles. Other data was collected by using a PH meter, SEM, X-ray diffraction, TEM, and AFM. | |||
Last, zein protein film formation was carried out using the BSA conjugated nanoparticles. First, a clear solution of zein (10%w/v) in aqueous ethanol (90%v/v) along with glycerol (30% on zein weight basis) was prepared. The BSA conjugated nanoparticles (10%v/v) was then added to this solution. Five grams of this solution was placed in a petri dish and swirled to coat the dish. It was placed in an 40˚C oven for 24 hours to eventually lead to protein film formation. A texture analyzer was then used in order to determine the mechanical properties of the films containing different samples of BSA conjugated Au nanoparticles (“Protein Films of Bovine Serum Albumen Conjugated Gold Nanoparticles: A Synthetic Route from Bioconjugated Nanoparticles to Biodegrable Protein Films” by Mandeep Singh Bakshi, Harpreet Kaur, Poonam Khullar, Tarlok Singh Banipa, Gurinder Kaur, and Narpinder Singh). | |||
==Data== | ==Data== | ||
Revision as of 14:06, 4 September 2011
 Biomaterials Design Lab
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ObjectiveThe purpose this experiment is to determine the ideal pH for the synthesis of Au(0) nanoparticles. For this experiment, Au(0) nanoparticles will be created by reducing HAuCl4 with bovine serum albumen (BSA) in an aqueous solution with a pH of 7.55. DescriptionProtocol: Materials needed included Chloroauric acid HAuCl4, bovine serum albumen (BSA), and zein protein. BSA(0.0015-0.015mM) and HAuCl4(0.25-10mM) were used to create a 10 mL aqueous mixtures. Each mixture was transferred to a screw-capped glass bottle. The mixtures were then kept in a water thermostat bath at 40, 60, or 80±1˚C for 6 hours. The solutions color changed from colorless to pink-purple and final purple. The samples were then removed from the bath and left to cool overnight. They were purified with distilled water two times in order to remove any excess BSA, using a centrifuge at 10000-14000rpm for 5 minutes. In order to determine the absorbance caused by surface plasmon resonance, UV-visible spectra were taken of each sample using an UV Spectrophotometer with a wavelength range of 200 to 900 nm. Some time dependent scans were also taken to understand the growth kinetics of Au nanoparticles. Finally, samples were placed in different water temperature baths (20 to 70˚C) to observe how temperature affects the synthesis of nanoparticles. Other data was collected by using a PH meter, SEM, X-ray diffraction, TEM, and AFM. Last, zein protein film formation was carried out using the BSA conjugated nanoparticles. First, a clear solution of zein (10%w/v) in aqueous ethanol (90%v/v) along with glycerol (30% on zein weight basis) was prepared. The BSA conjugated nanoparticles (10%v/v) was then added to this solution. Five grams of this solution was placed in a petri dish and swirled to coat the dish. It was placed in an 40˚C oven for 24 hours to eventually lead to protein film formation. A texture analyzer was then used in order to determine the mechanical properties of the films containing different samples of BSA conjugated Au nanoparticles (“Protein Films of Bovine Serum Albumen Conjugated Gold Nanoparticles: A Synthetic Route from Bioconjugated Nanoparticles to Biodegrable Protein Films” by Mandeep Singh Bakshi, Harpreet Kaur, Poonam Khullar, Tarlok Singh Banipa, Gurinder Kaur, and Narpinder Singh). Data
NotesThis area is for any observations or conclusions that you would like to note.
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